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Welcome to the MIT BioMicro Center!

* [[BioMicroCenter:Forms#NEW_USERS|New User Signup Forms]] * [[BioMicroCenter:Forms|External Submission Forms]] * [[BioMicroCenter:FAQ|FAQs]] |} The '''MIT BioMicro Center''' is an integrated genomics core facility that provides both expertise and equipment for systems biology. The core has significant resources in sample preparation, next generation sequencing and in high throughput screening as well as bioinformatics and BioIT. The Center is a joint endeavor between the [http://biology.mit.edu Department of Biology], the [http://ki.mit.edu Koch Institute for Integrative Cancer Research] (Integrated Genomics and Bioinformatics Core (IGB)), and the [http://web.mit.edu/be Department of Biological Engineering]. {| width="100%" |- valign="top" |colspan=2 align="center" style="background-color:yellow; border:1px solid purple"| {| |Assisted Services |} |align="center" style="background-color:aqua; border:1px solid black"| {| | Walkup Services |} |- |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"|Sample Isolation * Sonication ([[BioMicroCenter:Covaris|Covaris E220/R230]]) * DNA/RNA purification ([[BioMicroCenter:Tecan_Freedom_Evo#Revvity_Chemagic_360|Chemagic360]]) * Quality Control
([[BioMicroCenter:QC|AATI Fragment Analyzer/FemtoPulse]]) |} |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"|Library Preparation * [[BioMicroCenter:Illumina_Library_Preparation|Short Read Sample Preparation]] ** Standard Methods for [[BioMicroCenter:DNA_LIB| *DNA* ]] [[BioMicroCenter:RNA_LIB| *RNA* ]] ** [[BioMicroCenter:FAQ#STANDARD_versus_HIGH_THROUGHPUT_LIBRARY_PREPARATION|High Throughput Methods]] for [[BioMicroCenter:DNA_HTL| *DNA* ]] [[BioMicroCenter:RNA_HTL| *RNA* ]] * Long Read Sample Preparation
([[BioMicroCenter:NanoPore_Library_Prep|Nanopore]] and [[BioMicroCenter:PacBio_Library_Preparation|PacBio]]) |} |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"|Sample Quantification * Plate Readers ([[BioMicroCenter:Varioskan#Synergy_H1| Synergy H1]]/[[BioMicroCenter:Varioskan| Varioskan]]) * [[BioMicroCenter:RTPCR|RT-PCR (Roche LC480)]] * [[BioMicroCenter:QC| BioAnalyzer2100]] * [[BioMicroCenter:QC#NanoDrop_ND-1000| NanoDrop ND-1000]] |} |- |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"| [[BioMicroCenter:SingleCell|Single Cell]] and [[BioMicroCenter:SpTx|Spatial]] * 10X CHROMIUM
(5'RNA, 3'RNA, ATAC, multiome) * 10X VISIUM
(Standard, High Density) * ELEMENT AVITI24 (in situ prep/sequencing) |} |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"| [[BioMicroCenter:Sequencing|Sequencing Technologies]] * High Yield (>1b reads) **[[BioMicroCenter:Illumina_Sequencing|Illumina NovaSeqX (25b,10b)]]† * Mid Yield (250m-1b reads) **[[BioMicroCenter:Element Sequencing|Element AVITI24]] * Low Yield (1m-150m reads) **[[BioMicroCenter:Singular Sequencing|SingularG4]] **[[BioMicroCenter:Illumina_Sequencing|Illumina MiSeq i100]] * Long Read (1kb+) **[[BioMicroCenter:Oxford Nanopore Technologies|ONT PromethION]] **[[BioMicroCenter:PacBio|PacBio Revio]]† |} |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"|Automation / Library Prep * [[BioMicroCenter:Tecan Freedom Evo|Liquid Handlers]] ([[BioMicroCenter:Tecan_Freedom_Evo#JOHNNY_5|Tecan EVO]] * [[BioMicroCenter:Tecan_Freedom_Evo#SPT_LABTECH_MOSQUITO_HV|SPT Mosquito]]) * [[BioMicroCenter:Covaris|Covaris E220 and R230 Focused-ultrasonicators]] * [[BioMicroCenter:PippinPrep|Pippin Prep automated electrophoresis system]] * [[BioMicroCenter:Oligo_Synthesis| Oligo Synthesis]] |} |- |valign="top" colspan=3 align="center" style="background-color: lightsalmon; border:1px solid black"| {| |Informatics Services |} |- |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"|Data Analysis * [https://igb.mit.edu/ Bioinformatic Consulting] * [[BioMicroCenter:People#INFORMATICS_LAB|Informatics Staff]] |} |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"|BioIT/Research Computing * [https://koch-institute-mit.gitbook.io/mit-data-management-analysis-core/ FAIR Data Management] * [https://igb.mit.edu/computing-resources/active-data-storage Data Storage] * [https://igb.mit.edu/computing-resources/luria-cluster Computing on Luria] * [https://igb.mit.edu/data-management/globus Data Transfer (Globus)] |} |valign="top" align="center" style="background-color:transparent; border:1px solid black"| {| |width="10%" align="left"|Education/Training/Seminars * [https://igb.mit.edu/mini-courses Informatics/Computing Training Sessions] * [[BioMicroCenter:Technology_Seminar_Series|Technology Seminar Series]] * [http://mailman.mit.edu:/mailman/listinfo/biostuff Biostuff mailing list] |} |} † - service will be performed in a sister core facility (Harvard, DFCI, or Broad)

[https://wikis.mit.edu/confluence/display/bmc ''BMC Internal wiki'']

CONTACT US

[http://whereis.mit.edu 68-322]
biomicro@mit.edu
617-715-4533

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