# Workshop-April-2025 – Cryo-EM

**Source**: https://cryoem.iitm.ac.in/workshop-april-2025/
**Parent**: https://cryoem.iitm.ac.in/completed-workshops/

## Hands-On Workshop on Single Particle Analysis

- April 2-4, 2025

- ANRF National Facility for Cryo-Electron Microscopy, IIT Madras

This three-day workshop is designed to provide participants with in-depth knowledge and practical skills in cryo-electron microscopy (cryo-EM), with a focus on single-particle analysis (SPA). The event will feature a combination of lectures and hands-on sessions, enabling attendees to master techniques required for high-resolution structure determination. Whether you are a beginner or looking to refine your skills, this workshop will equip you with the tools and expertise to advance your research in structural biology.

## Key Highlights

The workshop will be a three-day intensive program hosted at the ANRF National Facility for Cryo-Electron Microscopy at IIT Madras. It will focus on the fundamentals and practical applications of single particle reconstruction, including:

- **Dates:** April 2-4, 2025
- **Venue:** ANRF National Facility for Cryo-Electron Microscopy, IIT Madras
- **Format:** Lectures and hands-on training sessions
- **Target Audience:** Researchers, students, and professionals in structural biology and related fields
- **Seats:** Limited (selection process detailed below)

## Instructors

### [Mamata Bangera](https://sites.google.com/smail.iitm.ac.in/bangeralab)

Assistant Professor \
IIT Madras\
Website: [Click here to view](https://sites.google.com/smail.iitm.ac.in/bangeralab)

### [Saikat Chowdhury](https://sites.google.com/view/sclab-at-ccmb/contact?authuser=0)

Senior Scientist\
CSIR-CCMB\
Website: [Click here to view](https://sites.google.com/view/sclab-at-ccmb/contact?authuser=0)

### [Somnath Dutta](https://somnath92.wixsite.com/website)

Associate Professor\
IISc\
Website: [Click here to view](https://somnath92.wixsite.com/website)

### [Sandip Kaledhonkar](https://www.bio.iitb.ac.in/people/faculty/kaledhonkar-s/)

Assistant Professor\
IIT Bombay\
Website: [Click here to view](https://www.bio.iitb.ac.in/people/faculty/kaledhonkar-s/)

### [Sucharita Bose](https://ecryom.instem.res.in/people)

Facility In-Charge\
DBT-InStem\
Website: [Click here to view](https://ecryom.instem.res.in/people)

### [Vidya Mangala Prasad](https://sites.google.com/view/vmprasadlab-iisc/home)

Assistant Professor\
IISc\
Website: [Click here to view](https://sites.google.com/view/vmprasadlab-iisc/home)

## Topics Covered

The workshop will provide hands-on training and theoretical insights into single-particle analysis (SPA) using cryo-electron microscopy. Key topics include:

## 1. Introduction to Single Particle Analysis

- Basics of SPA and its applications in structural biology
- Advantages of cryo-EM over traditional methods like X-ray crystallography
- Case studies showcasing SPA in macromolecular structure determination

## 2. Sample Preparation

- Protein/molecule preparation for cryo-EM
- Vitrification techniques for high-quality grid preparation
- Troubleshooting common issues in sample preparation

## 3. Data Acquisition

- Overview of cryo-TEM and direct electron detectors
- Best practices for image collection and motion correction techniques

## 4. Image Processing with RELION and CryoSPARC

- Particle picking and 2D classification
- 3D reconstruction workflows
- Iterative refinement for high-resolution structures

## 5. Addressing Challenges in SPA

- Managing sample heterogeneity and orientational bias
- Strategies for improving particle alignment and distribution

## 6. Validation and Interpretation

- Assessing resolution and map quality
- Biological interpretation of reconstructed models

## Learning Outcomes

By the end of the workshop, participants will:

- Gain a comprehensive understanding of the SPA workflow using **RELION** and **CryoSPARC**
- Acquire hands-on experience in **sample preparation, data processing,** and **3D reconstruction**
- Develop strategies to address **common challenges** in single-particle analysis

## Registration Process

Seats are limited, and participants will be selected based on their research interests.

## To Apply:

Applicants should send an email to **cryoem\_prg@icsrpis.iitm.ac.in** with a Word/PDF file containing the following details:

Name:\
Email ID:\
Affiliation (Institution, Name of Advisor, PhD/Postdoc, etc.)\
A 250-word essay on how cryo-electron microscopy (CryoEM) and single-particle analysis (SPA) are useful for your research.

Important Dates

- **Application Deadline:** February 28, 2025
- **Notification of Selection:** March 5, 2025
- **Payment Deadline:** March 8, 2025 (selected participants will receive a payment link)

Fees

- **Academic Participants:** ₹7,500
- **Industry Participants:** ₹15,000

The fee includes workshop registration, refreshments during the event, and accommodation (with breakfast, lunch, and dinner) at IIT Madras.

## Accommodation

Shared accommodation will be provided at **Taramani Guest House, IIT Madras campus.** Specific check-in/check-out details will be communicated to selected participants.

## Contact Us

For any queries regarding the workshop or registration process, please contact us during office hours (Monday–Friday, 9 AM – 5 PM IST):

- **Email:**  cryoem\_prg@icsrpis.iitm.ac.in
- **Phone:** 044-22574208