# Computational Structural Biology Lab

**Source**: http://www.csb.iitkgp.ac.in/applications/HotSPRing/main.php
**Parent**: http://www.csb.iitkgp.ac.in/

### Department of Biotechnology Indian Institute of Technology Kharagpur

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- Databases
  - [Protein folding similarity](#)
- Benchmarks
  - [Protein-RNA Docking Benchmark version 2](http://www.csb.iitkgp.ac.in/applications/PRDBv2.php)
  - [Protein-RNA Docking Benchmark version 1](http://www.csb.iitkgp.ac.in/applications/benchmark.php)
  - [Protein-RNA Affinity Benchmark](http://www.csb.iitkgp.ac.in/applications/affinity.php)
  - [ProRBR Dataset](http://www.csb.iitkgp.ac.in/applications/proRBR_dataset/proRBR)
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**HotSPRing:** **Hot** **S**pot at **P**rotein-**R**NA interact**ing** surface

- [Tutorial](http://www.csb.iitkgp.ac.in/applications/HotSPRing/docs/tutorial.php)
- [Instructions](http://www.csb.iitkgp.ac.in/applications/HotSPRing/docs/instructions.php)
- [References](http://www.csb.iitkgp.ac.in/applications/HotSPRing/docs/references.php)
- [Contact us](http://www.csb.iitkgp.ac.in/applications/HotSPRing/docs/contact.php)

Please submit the following: Items labelled (\*) are mandatory

|  |  |  |  |
| --- | --- | --- | --- |
| Protein-RNA complex file\* Submit a file in PDB format | Submit a file in PDB format | Protein chain ID(s)\* |  |
|  |  | RNA chain ID(s)\* |  |
| Please submit the [HSSP](http://mrs.cmbi.ru.nl/m6/) file\* |  | [HSSP](http://mrs.cmbi.ru.nl/m6/) chain ID(s)\* |  |
| *Unbound protein file **(Optional)*** |  | *Unbound chain ID(s)* |  |

This is a test run on Protein-RNA interface residues affinity prediction.

PDB IDs of files used are: 1DFU (bound), 1B75 (unbound).

You do not need to submit any PDB/HSSP file. Download link is also provided.