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Computational Structural Biology Lab

Source: http://www.csb.iitkgp.ac.in/applications/PRDBv2.php Parent: http://www.csb.iitkgp.ac.in/

Department of Biotechnology Indian Institute of Technology Kharagpur

Supplementary Information for Protein RNA docking benchmark Version 2

Each folder contains the protein-RNA complex, the unbound structure of protein and/or RNA (wherever available) along with the transformed coordinates of unbound protein and/or RNA subunits superposed with the bound subunits. The subunits have been filtered and only chains of interest are retained in the PDB files.

Click here to download the protein-RNA docking benchmark dataset. (Description of the dataset can be found here.)

Table 1: The benchmark dataset of protein-RNA complexes.

Bounda Unbound (PDB id)a Bb rmsd fnat GDT_TS LGA_S Category
PDB id Protein RNA Protein RNA c-rmsdc p-rmsdd i-rmsde
P Cα+P
I. Unbound-Unbound cases (21)
(A) Complexes with tRNA (10)
1ASY (A:R) S. cerevisiae aspartyl-tRNA synthetase tRNA-Asp 1EOV (A) 2TRA (A) 4430 1.5 4.9 1.3 3.3 2.3 34.0 85.4 92.8 R
1B23 (P:R) T. aquaticus EF-TU E. coli tRNA-Cys 1TUI (A) 1U0B* (A) 2616 10.0 7.2 13.3 13.0 13.2 19.0 50.3 47.7 X
1C0A (A:B) E. coliaspartyl-tRNA synthetase tRNA-Asp 1EQR (A) 1EFW* (C) 4504 1.6 1.8 1.6 1.8 1.7 70.8 84.0 92.0 S
1QTQ (A:B) E. coli glutaminyl-tRNA synthetase tRNA-Gln 1NYL (A) 3KNH* (Y) 5202 1.6 4.8 1.8 6.4 4.0 41.3 86.7 93.0 S
2BTE (A:B) T. thermophilus leucyl-tRNA synthetase tRNA-Leu 1OBC (A) 2NQP* (F) 3430 1.2 4.6 1.3 4.1 2.5 46.2 77.1 68.4 R
2DRB (A:B) A. fulgidus CCA-adding enzyme tRNAminiCCA 1UET (A) 3Q1Q* (C) 3198 1.1 3.4 1.8 4.2 2.5 22.0 86.8 87.8 S
2FMT (A:C) E. coli methionyl-tRNAfmet transformylase tRNAfmet 1FMT (A) 3CW6 (A) 2940 1.2 2.3 0.9 2.5 1.7 44.8 91.2 96.1 R
2ZZM (A:B) M. jannaschii tRNA (guanine(37)-N1)-methyltransferase Trm5b tRNA-Leu 2YX1 (A) 1WZ2* (C) 4523 15.1 4.6 20.0 6.4 12.9 43.6 74.5 73.9 X
3HL2 (AA'BB':E) H. sapiens selenium transferase tRNA-Sec 3BC8 (AA'A''A''') 3RG5 (A) 2186 0.6 5.2 1.3 6.0 4.3 39.4 98.0 98.9 R
3VJR (A:B) E. coli peptidyl-tRNA hydrolase tRNA CCA-acceptor 2PTH (A) 1NJM* (5) 1354 0.8 3.7 0.8 3.8 2.0 14.3 95.9 97.9 R
(B) Ribosomal proteins (3)
1DFU (P:MN) E. coli ribosomal protein L25 5s rRNA 1B75 (A) 364D (CB) 1690 3.0 5.3 3.0 4.4 3.7 14.3 73.4 70.4 X
1FEU (A:BC) T. thermophilus ribosomal protein L5 5s rRNA 2J01 (Z) 364D* (BC) 1595 2.1 3.3 2.4 2.8 2.6 55.1 82.1 80.7 S
1MMS (A:C) T. maritima ribosomal protein L11 23S rRNA fragment 2K3F (A) 3I9C* (A:1080-1129) 2455 3.5 1.6 4.1 1.6 3.3 35.6 65.2 62.3 X
(C) Duplex RNA (4)
1OOA (A:C) M. musculus NF-kappaB p50 aptamer RNA 1LE5 (B) 2JWV* (A) 1909 6.2 6.7 5.5 7.0 6.3 50.0 64.1 62.7 X
1R3E (A:C) T. maritime pseudouridine synthase TruB tRNA fragment 1ZE1 (A) 1EHZ (A:49-65) 3680 2.0 4.9 3.3 4.9 3.6 50.0 82.9 76.4 X
2R8S (LH:R) M. musculus synthetic FAB ribozyme 2HFF (AB) 1HR2 (A) 2510 1.6 4.3 2.9 2.1 2.5 50.9 87.1 93.8 S
3SNP (A:C) O. cuniculus iron regulatory protein 1 ferritin H IRE RNA 2B3Y (A) 1AQO* (A) 2872 0.7 3.8 14.5 3.3 12.8 31.3 44.2 40.2 X
(D) Single-stranded RNA (4)
1JBS (A:C) A. restrictus restrictocin SRD RNA analogue 1AQZ (A) 1Q9A (A) 1310 0.7 3.4 0.6 3.2 1.9 62.1 97.0 98.9 R
1WSU (A:E) M. thermoacetica elongation factor SelB SECIS RNA 1LVA (A) 2RLU (A) 940 0.7 4.2 0.5 1.1 0.8 73.7 98.0 98.5 R
1ZBH (AD:E) H. sapiens exonuclease ERI1 histone mRNA 1ZBU (AD) 1JU7 (A) 1760 0.6 3.6 0.6 3.4 2.0 72.7 49.6 49.8 R
2BH2 (A:C) E. coli methyltransferase RumA 23S rRNA 1UWV (A) 1JZX* (A:1915-1951) 4491 1.4 9.8 0.7 10.5 6.0 30.4 86.0 93.5 R
II. Unbound-Bound (95)
(A) Complexes with tRNA (16)
1F7U (A:B) S. cerevisiae arginyl-tRNA synthetase tRNA-Arg 1BS2 (A) 5767 2.0 2.2 79.2 75.1 S
1H3E (AA':B) T. thermophilus tyrosyl-tRNA synthetase tRNA-Tyr 1H3F (AB) 3758 7.7 4.5 68.3 62.6 X
1H4S (AB:T) T. thermophilus prolyl-tRNA synthetase tRNA-Pro 1HC7 (AB) 2480 1.0 0.9 88.6 94.5 R
1J1U (AA':B) M. jannaschii tyrosyl-tRNA synthetase tRNA-Tyr 1U7D (AB) 2240 1.3 0.8 73.5 71.6 R
1N78 (A:C) T. thermophilus glutamyl-tRNA synthetase tRNA-Glu 1J09 (A) 4510 1.9 1.4 86.4 92.4 R
1QF6 (A:B) E. coli threonyl-tRNA synthetase tRNA-Thr 1EVL (A) 4602 0.8 0.7 96.6 98.5 R
1SER (AB:T) T. thermophilus seryl-tRNA synthetase tRNA-Ser 1SES (AB) 2290 1.7 2.4 91.7 95.9 S
1U0B (B:A) E. coli cysteinyl-tRNA synthetase tRNA-Cys 1LI5 (B) 4560 0.7 1.0 97.7 98.9 R
1VFG (B:D) A. aeolicus CCA-adding enzyme tRNA 4WBY (A) 1664 1.6 1.4 90.8 95.1 R
2AZX (AA':C) H. sapiens tryptophanyl-tRNA synthetase tRNA-Trp 1R6U (AB) 2130 0.8 0.6 91.5 96.5 R
2FK6 (A:R) B. subtilis RNase Z tRNA-Thr 1Y44 (A) 1530 0.7 0.8 97.6 98.8 R
3ADB (A:C) M. jannaschii O-phosphoseryl-tRNA kinase tRNA-Sec 3A4M (A) 3099 22.4 27.3 72.8 70.2 X
3AMT (A:B) A. fulgidus agmatinylcytidine synthetase TiaS tRNA-Ile 3AU7 (A) 3821 0.8 0.7 95.6 93.6 R
4YCP (A:B) E. coli tRNA-dihydrouridine synthase C tRNA-Trp 4BFA (A) 2151 0.4 6.8 99.8 99.9 X
4YVJ (AB:C) H. influenzae tRNA methyltransferase D tRNA 3AXZ (AA') 2313 0.7 1.3 96.0 98.4 R
5HR7 (A:D) E. coli RNA methyltransferaseRlmN tRNA-Glu 3RFA (A) 3450 1.4 1.3 93.7 96.7 R
(B) Ribosomal proteins (2)
1SDS (C:FF') M. jannaschii 50S ribosomal protein L7Ae box H/ACA sRNA 1XBI (A) 1200 0.3 0.4 100.0 100.0 R
2HW8 (A:B) T. thermophilus ribosomal protein L1 mRNA 1AD2 (A) 2401 6.7 5.1 64.6 47.4 X
(C) Duplex RNA (32)
1MSW (D:R) Phage T7 RNA polymerase RNA transcript 1ARO (P) 1830 3.5 3.5 57.1 54.1 X
1N35 (A:BC) Reovirus polymerase dsRNA 1MUK (A) 3240 0.5 0.9 99.3 99.7 R
1Q2R (AB:E) Z. mobilis guanine transglycosylase 20-mer tRNA fragment 1R5Y (AA') 3006 0.6 1.6 98.0 99.0 S
1WNE (A:BC) Foot-and-mouth disease virus polymerase template-primer RNA decanucleotide 1U09 (A) 3080 0.7 0.6 97.2 99.0 R
1YVP (B:EFH) X. laevis Ro autoantigen Y RNA 1YVR (A) 4207 1.4 1.3 90.6 95.2 R
1ZBI (A:CD) B. halodurans RNase H A form RNA 1ZBF (A) 1675 0.6 0.6 98.7 99.5 R
2AZ0 (AB:CD) Flock house virus B2 siRNA 2B9Z (AB) 2264 1.3 1.0 85.5 93.3 R
2BGG (A:PQ) A. fulgidus PIWI siRNA 1W9H (A) 2240 0.9 0.8 96.5 98.2 R
2EZ6 (AB:CD) A. aeolicus RNase III 28-mer RNA 1JFZ (AB) 5193 1.0 0.8 95.5 84.9 R
2F8S (AB:CD) A. aeolicus argonaute siRNA 1YVU (A) 990 1.9 1.5 77.4 78.3 S
2GJW (AB:EFH) A. fulgidus splicing endonuclease bulge-helix-bulge RNA 1R0V (AB) 3243 1.0 1.0 83.0 91.7 R
2GXB (A:EF) H. sapiens Z alpha domain of adenosine deaminase dsRNA 1QGP (A) 768 1.1 0.7 87.9 90.9 R
2QUX (AB:C) Pseudomonas phage PP7 coat protein hairpin RNA 2QUD (AB) 1754 0.9 0.5 96.9 98.4 R
2Y8W (A:B) T. thermophilus endoribonuclease Cse3 hairpin RNA 1WJ9 (A) 3258 1.1 3.1 95.5 97.3 X
2ZKO (AB:CD) NS1 protein of influenza A A-form dsRNA 2Z0A (AB) 2466 0.6 0.8 97.7 98.8 R
3BSN (A:PT) Norwalk virus polymerase synthetic RNA 1SH3 (A) 3111 1.3 1.9 90.9 95.2 S
3DD2 (H:B) H. sapiens thrombin 26-mer RNA 1JOU (D) 1822 0.6 0.5 96.5 98.1 R
3EQT (AB:CD) H. sapiens helicase DHX58 dsRNA 2W4R (AA') 2705 1.2 1.2 47.9 42.5 R
3FTE (A:CD) A. aeolicus methyltransferase KsgA rRNA 3FTD (A) 836 1.4 1.1 89.7 94.9 R
3KS8 (AB:EF) R. ebolavirus polymerase cofactor VP35 dsRNA 3KS4 (AB) 1663 0.6 0.4 49.8 50.1 R
3MOJ (B:A) B. subtilis helicase dbpA E.coli 23S rRNA fragment 2G0C (A) 1758 1.0 1.0 94.5 97.6 R
3O3I (X:A) H. sapiens Hiwi1 PAZ domain piRNA 2L5C (A) 923 2.1 2.7 79.0 84.5 S
3OIJ (AB:C) S. cerevisiae methyltransferase SSU rRNA 3OII (AB) 2070 0.8 1.7 97.0 98.5 S
3OL6 (A:BCD) Poliovirus polymerase synthetic RNA 1RA6 (A) 4174 0.8 1.1 94.9 97.8 R
3RW6 (A:H) H. sapiens nuclear RNA export factor 1 CTE RNA 3RW7 (A) 2699 13.5 19.0 66.4 64.4 X
4ERD (AB:CD) T. thermophila p65 C-terminal domain stem IV of telomerase RNA 4EYT (AB) 3280 1.5 1.2 47.0 46.5 R
4FVU (A:BC) Lassa virus exonuclease dsRNA 3Q7C (A) 1240 1.0 0.7 97.2 98.5 R
4IG8 (A:BC) H. sapiens oligoadenylate synthetase 1 dsRNA 1PX5 (A) 2914 2.4 2.8 79.0 74.4 S
4ILL (AB:RC) S. solfataricus endoribonuclease Cas6 CRISPR RNA 4ILR (AA') 7074 0.9 0.9 96.3 98.1 R
4ZT0 (A:B) S. pyogenes CRISPR-associated endonuclease Cas9 single-guide RNA 4CMP (A) 10845 29.6 22.0 45.6 43.1 X
5ED1 (A:BC) H. sapiens ADAR2 S. cerevisiae dsRNA 1ZY7 (A) 2403 1.1 2.3 95.4 97.5 S
5F5H (A:C) M. musculus Roquin-1 Ox40 3'-UTR 4QI0 (A) 1618 0.5 0.6 99.2 99.6 R
(D) Single-stranded RNA (45)
1AV6 (A:B) Vaccinia virus cap-specific mRNA methyltransferase M7G capped RNA 1B42 (A) 844 0.5 0.4 99.1 99.6 R
1C9S (L:W1-7) G. stearothermophilusTRAP ssRNA 2EXT (A) 1027 0.3 0.2 100.0 100.0 R
1CVJ (A:M) H. sapiens poly(A)-binding protein polyA 4F25 (A) 2822 2.7 3.9 93.4 93.9 X
1G2E (A:B) H. sapiens RRM domain of the HuD protein class II ARE fragment 4EGL (A) 2776 0.9 0.6 49.8 49.8 R
1K8W (A:B) E. coli pseudouridine synthase B T stem-loop RNA 1R3F (A) 2973 1.9 1.3 80.8 90.3 R
1KQ2 (ABHIKM:R) S. aureus Hfq 7-mer RNA 1KQ1 (ABHIKM) 3026 0.6 0.5 81.9 91.8 R
1M5O (C:B) H. sapiens U1 Snp A hairpin ribozyme 1OIA (A) 1770 1.2 0.9 96.6 98.1 R
1M8V (AM:O) P. abyssi Sm uridine heptamer 1H64 (AM) 1290 0.6 0.4 62.2 61.2 R
1M8W (A:CE) H. sapiens pumilio-homology domain Nre1-19 RNA 1M8Z (A) 2110 0.8 0.7 90.9 96.0 R
1UVI (A:D) phi6 RNA polymerase 6-mer RNA 1HHS (A) 1814 0.2 0.2 100.0 100.0 R
1WPU (A:C) B. subtilis HutP antitermination protein hut mRNA 1WPV (A) 1360 0.2 0.2 100.0 100.0 R
2A8V (B:E) E. coli RHO Cytosine rich RNA 1A8V (B) 720 1.0 1.6 94.7 97.7 S
2ASB (A:B) M. tuberculosis Nus A antiterminator BoxC rRNA 1K0R (A) 2320 1.1 0.8 91.8 96.4 R
2GIC (A:R) VSV nucleocapsid viral genomic RNA 2QVJ (A) 2000 1.3 0.9 91.9 95.7 R
2IX1 (A:B) E. coli RNase II polyA 2IX0 (A) 4160 1.6 0.9 86.2 93.3 R
2JEA (AB:C) S. solfataricus exosome substrate RNA 2JE6 (AB) 1533 0.3 0.3 99.8 99.9 R
2JLU (A:C) Dengue virus serine protease subunit NS3 ssRNA 2JLQ (A) 1925 2.1 2.8 74.3 78.1 S
2PY9 (B:E) H. sapiens poly(rC)-binding protein 2 human telomeric RNA 2JZX (A:1-73) 1060 1.4 1.6 85.5 92.0 R
2Q66 (A:X) S. cerevisiae poly(A) polymerase polyA 2HHP (A) 1811 1.9 2.1 85.0 91.5 S
2R7R (A:X) Rotavirus polymerase VP1 RNA 2R7Q (A) 1955 0.2 1.9 98.7 99.3 S
2XGJ (A:C) S. cerevisiae Mtr4 polyA 4U4C (A) 1389 4.1 3.1 71.5 65.5 X
2XNR (A:C) S. cerevisiae Nab3-RRM UCUU recognition sequence 2XNQ (A) 926 1.0 1.2 94.3 97.4 R
2XZO (A:D) H. sapiens Upf1 helicase polyU 2XZP (A) 2006 6.2 5.0 57.2 54.2 X
3AEV (B:C) P. horikoshii Dim2p P. horikoshii 16S rRNA fragment 2E3U (A) 2416 0.7 0.5 97.4 99.0 R
3BX2 (A:C) S. cerevisiae PUF4 3' UTR binding sequence 3BWT (A) 2467 0.6 0.5 98.5 99.3 R
3D2S (A:E) H. sapiens MBNL1 ZnF3/4 pre-mRNA 3D2Q (A) 569 0.8 0.6 97.0 98.7 R
3MDG (AB:C) H. sapiens CFI(m)25 pre-mRNA 3BAP (AA') 1069 0.5 0.9 96.5 98.1 R
3NMR (A:B) H. sapiens CUG-binding protein 1 UGU-rich mRNA 2DHS (A) 1096 2.7 2.2 43.0 39.7 S
3O8C (A:C) HCV NS3 helicase polyU 3O8B (A) 1909 1.0 1.1 94.6 97.5 R
3PF4 (B:R) B. subtilis CspB ssRNA 1CSP (A) 963 0.5 0.4 98.5 99.4 R
3QJJ (A:Q) P. horikoshii RAMP Protein CRISPR repeat RNA 3UFC (X) 3191 1.0 1.0 96.0 98.3 R
3RC8 (A:E) H. sapiens helicase SUPV3L1 RNA fragment 3RC3 (A) 1471 1.1 0.8 92.3 96.3 R
4E78 (A:PT) HCV polymerase RNA primer-template pair 4E76 (A) 2100 0.7 0.8 98.4 99.4 R
4H5P (AB:E) Rift valley fever virus nucleocapsid polyU 3LYF (AB) 4482 0.9 2.1 42.5 42.6 S
4HOR (A:X) H. sapiens IFIT polyC 4HOQ (A) 1747 3.1 2.4 68.7 72.5 S
4J7M (A:B) M. musculus Dom3Z polyU 4J7N (A) 1268 0.3 0.3 99.7 99.9 R
4M59 (AB:CD) Z. mays chloroplast ppr10 psaJ RNA 4M57 (AA') 7842 10.5 7.1 30.1 26.1 X
4MDX (AB:C) B. subtilis mRNA interferase MazF mRNA 1NE8 (AA') 2127 0.7 1.6 98.4 86.4 S
4N2Q (A:B) B. distachyon thylakoid assembly 8 Zm4 13-mer RNA 4ME2 (A) 814 2.3 1.3 78.6 82.3 R
4QU6 (A:B) H. sapiens GRSF1 synthetic RNA 2LMI (A) 696 2.5 2.4 80.8 80.6 S
5BUD (A:B) C. albicans decapping nuclease RAI1 polyU 5BTH (A) 1262 0.5 1.2 99.5 99.8 R
5DET (AB:PQ) H. sapiens RBPMS CAC containing ssRNA 5CYJ (AB) 2137 1.2 1.6 46.9 47.6 S
5EIM (A:C) S. pombe YTH domain of spMmi1 synthetic RNA 5H8A (A) 1529 0.6 1.3 98.2 99.3 R
5ELS (AC:HI) H. sapiens KH domain of T-STAR synthetic RNA 5EL3 (AB) 2313 1.5 2.9 47.7 49.1 S
5EX7 (A:B) D. melanogaster Brat NHL domain hunchback mRNA 4ZLR (A) 1639 0.4 0.6 99.8 99.9 R
III. Bound-Unbound cases (10)
(A) Complexes with tRNA (2)
2ZM5 (A:C) E. coli iso-pent-enyl-transferase tRNA-Phe 3L0U (A) 3936 4.2 6.5
3ZJT (A:B) E. coli leucyl-tRNA synthetase tRNA-Leu 2NQP (F) 4322 2.8 2.9
(C) Duplex RNA (5)
1HQ1 (A:B) E. coli SRP 4.5S RNA domain IV 1DUH (A) 1364 38.3 30.4
1JID (A:B) H. sapiens SRP19 SRP RNA 1D4R (A) 1436 2.6 4.0
2OZB (AB:C) H. sapiens ribonucleoprotein U4snRNA 2XEB (AB) 2569 11.0 12.5
2PJP (A:B) E. coli SelB SECIS RNA 1MFK (A) 1300 2.5 3.6
3BT7 (A:C) E. coli methyltransferase TrmA T-arm analogue 1EVV (A) 2230 3.1 3.5
(D) Single-stranded RNA (3)
1LNG (A:B) M. jannaschii SRP19 7S SRP RNA 1Z43 (A) 2367 2.0 2.1
2I82 (A:E) E. coli pseudouridine synthase RluA tRNA-Phe 3L0U (A:26-43) 3018 4.2 4.7
3IEV (A:D) A. aeolicus GTPase era 16S rRNA 1SDR (B) 2273 7.6 8.4

aFour letter PDB code of the protein-RNA complexes used in the benchmark with the chain id of the protein and the RNA molecules in the parenthesis. For structures solved by NMR (shown in bold) the closet model to the average structure of the ensemble was considered as the reference structure for structural alignment. Symmetry related chains are primed (For example, A' and B' in 3HL2). If the Unbound RNA structure is taken from another complex they are marked with * (For example, in 3I9C nucleotides from 1080 to 1129 are considered).

bSurface area buried between protein and RNA upon complexation.

Crmsd calculated considering the equivalent Cα atoms.

drmsd calculated considering the equivalent P atoms.

ermsd calculated considering the equivalent interface atoms.