# Computational Structural Biology Lab

**Source**: http://www.csb.iitkgp.ac.in/applications/proRBR/mannual.php
**Parent**: http://www.csb.iitkgp.ac.in/applications/proRBR/index.php

### Department of Bioscience and Biotechnology Indian Institute of Technology Kharagpur

- [Home](http://www.csb.iitkgp.ac.in/)
- [Research](http://www.csb.iitkgp.ac.in/pages/research.php)
- [Publications](http://www.csb.iitkgp.ac.in/pages/publications.php)
- [Teaching](http://www.csb.iitkgp.ac.in/pages/teaching.php)
- Tutorials
  - [Python Tutorial](http://www.csb.iitkgp.ac.in/tutorials/python/python_index.php)
  - [Gromacs Tutorial](http://www.csb.iitkgp.ac.in/tutorials/gromacs/gromacs_index.php)
- Tools
  - [PRince](http://www.facweb.iitkgp.ac.in/~rbahadur/prince/home.html)
  - [Layers](http://www.csb.iitkgp.ac.in/applications/mol_layers/main.php)
  - [HotSPRing](http://www.csb.iitkgp.ac.in/applications/HotSPRing/main.php)
  - [NCodR](http://www.csb.iitkgp.ac.in/applications/NCodR/index.php)
  - [Nucpred](http://www.csb.iitkgp.ac.in/applications/Nucpred/index.php)
  - [pmiRScan](http://www.csb.iitkgp.ac.in/applications/pmiRScan/index.php)
  - [proRBR](http://www.csb.iitkgp.ac.in/applications/proRBR/index.php)
  - [Rotamers for RBPs](http://www.csb.iitkgp.ac.in/applications/RBP-Rotamer/rotamer.php)
  - [MetIoR](http://www.csb.iitkgp.ac.in/applications/MetIoR/index.php)
- Databases
  - [PMMLID](http://www.csb.iitkgp.ac.in/databases/miR_details/index.php)
  - [TFNRDv1.0](http://www.csb.iitkgp.ac.in/databases/TFNRDv1.0/tfnrd.html)
  - [ProRBR Dataset](http://www.csb.iitkgp.ac.in/applications/proRBR_dataset/proRBR.php)
- Benchmarks
  - [Protein-RNA Docking Benchmark version 3](http://www.csb.iitkgp.ac.in/applications/PRDBv3/PRDBv3.php)
  - [Protein-RNA Docking Benchmark version 2](http://www.csb.iitkgp.ac.in/applications/PRDBv2.php)
  - [Protein-RNA Docking Benchmark version 1](http://www.csb.iitkgp.ac.in/applications/benchmark.php)
  - [Protein-RNA Affinity Benchmark](http://www.csb.iitkgp.ac.in/applications/PRDBv3/Affinity.php)
- [Members](http://www.csb.iitkgp.ac.in/pages/members.php)
- Links
  - [Bioinformatics Centre](http://www.csb.iitkgp.ac.in/bioinformatics_centre/index.html)
  - [Homepage IITKGP](http://www.iitkgp.ac.in)
  - [Homepage Department of Biotechnology](http://www.bt.iitkgp.ac.in)
- [Contact](http://www.csb.iitkgp.ac.in/pages/contact.php)

# **proRBR** A Protein-RNA Binding Residues Predictor

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#### proRBR server allows user to predict Protein-RNA Binding Residues (RBRs) and non-Protein-RNA Binding Residues (non-RBRs) for a given protein sequence. The model achieved prediction accuracy of 86% and Area under the curve (AUC) of 0.93.

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### What is "proRBR"? [[Go to application]](http://www.csb.iitkgp.ac.in/applications/proRBR/index.php)

#### proRBR-Manual

1. Either type or paste the input protein sequence in FASTA format or upload a FASTA file using the Browse button.

Note that the input sequence should be a protein sequence with standard amino acids. The input can be a single line sequence or a single-line description followed by line of sequence.

2. Click on the submit button to run the prediction. The webserver uses the default RF-NC-triplet model for prediction, once the user submit the query sequence.
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3. Users can optionally select other prediction models such as RF-NC-quartet, SVMFBF, or KNN developed using quartet nucleotide composition feature and other optimized ML algorithms using the select prediction model button.
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4. On submitting the query sequence, the output of prediction result is displayed on the screen. Each nucleotide is assigned a prediction annotation (1/0) for protein-binding and non-binding nucleotides along with their raw probability scores. Optionally, user can download the prediction results by clicking on the Download here link.
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5. The Test Run button allows user to check an example (test case) input sequence and the corresponding prediction results.
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### [[Go to application]](http://www.csb.iitkgp.ac.in/applications/proRBR/index.php)