# Yale University

**Source**: https://cbic.yale.edu/instruments-listing?field_instrument_type_value=Solution+Biophysics
**Parent**: https://cbic.yale.edu/instruments

# Instrument Catalog

Instrument Type 

- Any -Diffraction (X-ray and electron)ImagingMass SpectrometryNMR & EPROptical SpectroscopyScatteringSolution BiophysicsStructural Science

Instrument Location 

- Any -CRB 100KCL 3KCL 7KCL 11KCL 12KCL 16BSCL 148SCL 136SCL 123SCL 123aSCL 26

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Analytical Ultracentrifuge Beckman Coulter Proteomelab XL-I

**Short Name:** AU

**Instrument Type:** Solution Biophysics

**Instrument Location:** KCL 3

**Date of Acquisition:** November 2009

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Analytical ultracentrifugation is a column-free separation technique that measures the relative change in the distribution of molecular weights, providing a way to measure heterogeneity, stoichiometry and self-association of proteins, oligomers, aggregates, particles, colloids, and small structures in solution.

Applied Photophysics Chirascan Circular Dichroism Spectrometer

**Short Name:** CD

**Instrument Type:** Solution Biophysics

**Instrument Location:** KCL 3

**Date of Acquisition:** September 2013

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Chirascan is a modern, high performance circular dichroism (CD) spectrometer operating in the ultraviolet and visible wavelength range. It is typically used to probe the secondary structure or conformation of bio-molecules.

[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/CD%20SOP.pdf)

DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II

**Short Name:** DLS

**Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science

**Instrument Location:** KCL 3

**Date of Acquisition:** October 2013

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Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.

Typical applications for DLS include:

- Screening macromolecules prior to crystallization trials
- Measuring the stability of protein samples for NMR analysis
- Monitoring molecular aggregation
- Characterizing nanoparticles

The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.

[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/DLS%20SOP.pdf)

TA NanoITC

**Short Name:** ITC

**Instrument Type:** Solution Biophysics

**Instrument Location:** KCL 16B

**Date of Acquisition:** August 2014

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By determining heat changes associated with binding between any two biomolecules, ITC measures all binding parameters in a single label-free, in-solution experiment.

TCSPC TD-Fluor Horiba Fluorolog 3 Time Domain Fluorimeter

**Short Name:** TCSPC

**Instrument Type:** Solution Biophysics

**Instrument Location:** KCL 16B

**Date of Acquisition:** December 2009

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The Fluorolog 3 is a modular fluorimeter that can be set up to make time-domain fluorescence measurements at specific wavelengths, or steady-state fluorescence measurements over a range of excitation and/or emission wavelengths.

Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System

**Short Name:** SAXSWAXS

**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science

**Instrument Location:** SCL 26

**Date of Acquisition:** December 2022

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#### The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.

In particular, the following key features enable the user to optimize experiments or results:

- High flux settings adapted for fast kinetics embedded in a low background camera
- Largest surface of detection moveable all the way from WAXS to long distance SAXS to optimize resolution and signal-to-noise
- Long distance SAXS settings for measuring large characteristic dimensions (up to 900 nm depending on the Xeuss 3.0 model)
- Optional USAXS module to characterize  large structures ( > 2.5 µm)