# Yale University
**Source**: https://cbic.yale.edu/instruments-view/a500a
**Parent**: https://cbic.yale.edu/instruments/experiment-runtime-estimation
# A500a Agilent DD2 500 MHz NMR Spectrometer (Manual)
Short Name:
A500a
Instrument Type:
NMR & EPR
Location:
KCL 3
Date of Acquisition:
January 2014
Description:
2 channel system, OneNMR probe with auto-tune-and-match installed.
[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)
Best experiments:
- PROTON
- COSY
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
- Variable Temperature experiments
- Kinetics experiments
Acceptable experiments:
- C13CPD
Not available:
- Simultaneous F19 and H1 experiments
**Time Required for NMR Experiments on A500a:**
| | | | | | | |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 5 hours | 16962 hours | 67 h 16 min | 820 h 42 min | 3113 h 37 min |
| 0.06 mg | 0.2 mM | 1 h 10 min | 424 h 02 min | 16 h 50 min | 205 h 15 min | 779 h 06 min |
| 0.15 mg | 0.5 mM | 0 h 11 min | 678 h 28 min | 2 h 45 min | 32 h 50 min | 124 h 34 min |
| 0.3 mg | 1 mM | 0 h 03 min | 170 h 00 min | 0 h 42 min | 8 h 20 min | 31 h 09 min |
| 0.6 mg | 2 mM | < 1 min | 4 h 15 min | 0 h 11 min | 2 h 05 min | 7 h 48 min |
| 1.5 mg | 5 mM | < 1 min | 3 h 11 min | 0 h 03 min | 0 h 08 min | 1 h 40 min |
| 3 mg | 10 mM | < 1 min | 1 h 42 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 25 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 06 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
Instrument Image: