Yale University

Source: https://cbic.yale.edu/instruments-view/a400c Parent: https://cbic.yale.edu/instruments/experiment-runtime-estimation

A400c Agilent DD2 400 MHz NMR Spectrometer (Autosampler)

Short Name:

A400c

Instrument Type:

NMR & EPR

Location:

KCL 3

Date of Acquisition:

October 2013

Description:

2 channel system with AS7620 96-sample changer. OneNMR probe with auto-tune-and-match installed.

Primarily used for short, routine NMR experiments where data is needed quickly.

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A400c:


Mass	Concentration	Proton	Carbon	gCOSY	gHSQC	gHMBC
0.03 mg	0.1 mM	10 h 40 min	35389 hours	1785 hours	1886 hours	7129 hours
0.06 mg	0.2 mM	2 h 40 min	10516 hours	46 h 28 min	474 h 12 min	1785 hours
0.15 mg	0.5 mM	0 h 26 min	1415 h 33 min	6 h 10 min	75 h 01 min	284 h 44 min
0.3 mg	1 mM	0 h 06 min	354 h 01 min	1 h 40 min	18 h 53 min	71 h 19 min
0.6 mg	2 mM	0 h 01 min	88 h 31 min	0 h 26 min	4 h 42 min	17 h 51 min
1.5 mg	5 mM	< 1 min	14 h 09 min	0 h 03 min	0 h 45 min	2 h 51 min
3 mg	10 mM	< 1 min	3 h 32 min	0 h 03 min	0 h 16 min	1 h 07 min
6 mg	20 mM	< 1 min	0 h 53 min	0 h 03 min	0 h 08 min	0 h 17 min
12 mg	40 mM	< 1 min	0 h 13 min	0 h 03 min	0 h 08 min	0 h 17 min
15 mg	50 mM	< 1 min	0 h 08 min	0 h 03 min	0 h 08 min	0 h 17 min
> 30 mg	100+ mM	< 1 min	0 h 04 min	0 h 03 min	0 h 08 min	0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

Instrument Image: