Yale University

Source: https://cbic.yale.edu/instruments-view/a400b Parent: https://cbic.yale.edu/instruments/experiment-runtime-estimation

A400b Agilent DD2 400 MHz NMR Spectrometer (Manual)

Short Name:

A400b

Instrument Type:

NMR & EPR

Location:

KCL 3

Date of Acquisition:

October 2013

Description:

2 channel system. AutoX DB probe installed. Variable-temperature and H/F experiments available.

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A400b:


Mass	Concentration	Proton	Carbon	gCOSY	gHSQC	gHMBC
0.03 mg	0.1 mM	44 h 44 min	50960 hours	644 hours	7862 hours	29844 hours
0.06 mg	0.2 mM	11 h 11 min	12740 hours	161 hours	1965 hours	7461 hours
0.15 mg	0.5 mM	1 h 47 min	2038 h 24 min	25 h 47 min	319 h 18 min	1193 h 57 min
0.3 mg	1 mM	0 h 27 min	509 h 36 min	6 h 30 min	78 h 37 min	298 h 40 min
0.6 mg	2 mM	0 h 07 min	127 h 27 min	1 h 38 min	19 h 48 min	75 h 40 min
1.5 mg	5 mM	0 h 01 min	20 h 23 min	0 h 16 min	3 h 08 min	12 h 15 min
3 mg	10 mM	< 1 min	5 h 15 min	0 h 06 min	0 h 47 min	1 h 32 min
6 mg	20 mM	< 1 min	1 h 16 min	0 h 03 min	0 h 08 min	0 h 17 min
12 mg	40 mM	< 1 min	0 h 19 min	0 h 03 min	0 h 08 min	0 h 17 min
15 mg	50 mM	< 1 min	0 h 12 min	0 h 03 min	0 h 08 min	0 h 17 min
> 30 mg	100+ mM	< 1 min	0 h 04 min	0 h 03 min	0 h 08 min	0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

Instrument Image: