# Zahabyia Malubhoy

**Source**: https://cbr.cs.tum.de/en/team/personnel/zahabyia-malubhoy
**Parent**: https://cbr.cs.tum.de/en/team/personnel

**Curriculum vitae**

- Since 2023: PhD at the Chair of Chemistry of Biogenic Resources
- 2020 - 2022: Master's degree in Chemical Biotechnology, Technical University of MunichMaster thesis: Cell-free biocatalytic production of alpha-ketoglutarate from galacturonate via an enzymatic cascade reaction, Chair of Chemistry of Biogenic Resources, TU Munich
- 2016 - 2019: Bachelor studies in Biochemistry and Cell Biology, Jacobs University Bremen
- 09/2018 - 12/2018: Exchange semester, University of Aberdeen

**Research**

Project name: VIENNA (*Vibrio natriegens* as a synthetic biology platform)

Project partners: Chair for Microbial Industrial Biotechnology (Prof. Blombach, TUMCS), WACKER Chemie AG

Project description:

*Vibrio natriegens* is a Gram negative bacterium with the highest growth rate described under laboratory conditions, with a doubling time of less than 10 mins. This extraordinary growth rate has generated a lot of interest in this microbe in the past few years. One of the objectives of my PhD project is to develop a high-yielding cell-free protein expression platform based on *Vibrio natriegens –* it’s natural capacity for superfast growth should theoretically make it one of the best yielding CFPS platforms and I aim to unlock this potential during my work. This objective will be achieved in close cooperation with the Chair for Microbial Biotechnology who will develop new *V. natriegens* chassis strains. This part of the project provides a combination of molecular biology and machine learning to develop a high performing in vitro system.

The cell-free protein expression platform will then be used for the expression of enzymes which can be freely mixed together to quickly prototype enzyme cascades at a microliter scale. The overarching objective is to accelerate the creation of high-performing enzymatic routes from substrates to products through cell-free prototyping. This aspect of the project involves enzyme assays and the development of automated workflows to develop a platform suitable for broad range prototyping projects.