# Concentrations of available and total nitrogen, formylated phloroglucinol compounds and unsubstituted B-ring flavanones in eucalypt foliage (mature phase and epicormic growth) and how it changes over time, 2020

**Source**: https://datacommons.anu.edu.au/DataCommons/rest/display/anudc:6457?layout=def:display
**Parent**: https://datacommons.anu.edu.au/DataCommons/

This data looks at how eucalypt leaf chemistry changes over time in a burnt and unburnt landscape, observing changes in different leaf types, including adult phase foliage in a burnt area, adult phase foliage in an unburnt area and epicormic growth in a burnt area. Total and available nitrogen and two plant secondary metabolites known to deter marsupial folivore feeding behaviour were also measured, including formylated phloroglucinol compounds (FPCs) and unsubstituted B-ring flavanones (UBFs). This study was undertaken to look at how fire can impact leaf chemistry, the changes in chemistry over time and what this may mean for marsupial folivores living in a burnt landscape, i.e., how the quality of the food source changes after fire and as a landscape recovers. To obtain this data, we randomly sampled 10 trees from each of the six eucalypt species present at both the burnt and unburnt sites. Leaf collection commenced in November 2020 and ended in November 2021, with sampling occurring every 4-6 weeks, with a total of seven samplings rounds being completed. At the start of the first collection round, the GPS location of each tree was recorded and we measured the diameter breast height (DBH). Epicormic growth was collected in the burnt landscape (and we opportunistically collected adult phase foliage where it was available) and adult phase foliage in the unburnt landscape was collected. Adult phase foliage was collected using an arborist throw-line launcher. We collected approximately 100g of leaf per tree by removing them from the stems. They were frozen at -20°C at the end of each day. all samples were freeze dried and ground and then we collected reflectance spectrum using a lab-based near-infrared reflectance spectrometer. We could then calculate total and available nitrogen using a Leco Truspec C/N analyser. UBFs and FPCs were quantified using High-Performance Liquid Chromatography.

Type

collection

Title

Concentrations of available and total nitrogen, formylated phloroglucinol compounds and unsubstituted B-ring flavanones in eucalypt foliage (mature phase and epicormic growth) and how it changes over time, 2020 - 2021

Brief Title

Temporal changes in eucalypt leaf chemistry over time

Collection Type

Dataset

Access Privileges

Research School of Biology

DOI - Digital Object Identifier

10.25911/zymd-rk43

Metadata Language

English

Data Language

English

Significance Statement

One of the first studies to look at the chemical composition of eucalypt epicormic foliage after fire and it changes across time. It also measures leaf chemistry changes in adult phase foliage in a burnt and unburnt landscape so comparisons can be drawn.

Brief Description

This data looks at how eucalypt leaf chemistry changes over time in a burnt and unburnt landscape, observing changes in different leaf types, including adult phase foliage in a burnt area, adult phase foliage in an unburnt area and epicormic growth in a burnt area. Total and available nitrogen and two plant secondary metabolites known to deter marsupial folivore feeding behaviour were also measured, including formylated phloroglucinol compounds (FPCs) and unsubstituted B-ring flavanones (UBFs).

Full Description

This data looks at how eucalypt leaf chemistry changes over time in a burnt and unburnt landscape, observing changes in different leaf types, including adult phase foliage in a burnt area, adult phase foliage in an unburnt area and epicormic growth in a burnt area. Total and available nitrogen and two plant secondary metabolites known to deter marsupial folivore feeding behaviour were also measured, including formylated phloroglucinol compounds (FPCs) and unsubstituted B-ring flavanones (UBFs). This study was undertaken to look at how fire can impact leaf chemistry, the changes in chemistry over time and what this may mean for marsupial folivores living in a burnt landscape, i.e., how the quality of the food source changes after fire and as a landscape recovers. To obtain this data, we randomly sampled 10 trees from each of the six eucalypt species present at both the burnt and unburnt sites. Leaf collection commenced in November 2020 and ended in November 2021, with sampling occurring every 4-6 weeks, with a total of seven samplings rounds being completed. At the start of the first collection round, the GPS location of each tree was recorded and we measured the diameter breast height (DBH). Epicormic growth was collected in the burnt landscape (and we opportunistically collected adult phase foliage where it was available) and adult phase foliage in the unburnt landscape was collected. Adult phase foliage was collected using an arborist throw-line launcher. We collected approximately 100g of leaf per tree by removing them from the stems. They were frozen at -20°C at the end of each day. all samples were freeze dried and ground and then we collected reflectance spectrum using a lab-based near-infrared reflectance spectrometer. We could then calculate total and available nitrogen using a Leco Truspec C/N analyser. UBFs and FPCs were quantified using High-Performance Liquid Chromatography.

Contact Email

murraya.lane@anu.edu.au

Contact Address

134 Linnaeus Way, Acton ACT 2601

Contact Phone Number

0432291303

Principal Investigator

Murraya Lane

Supervisors

Karen Marsh;
Kara Youngentob;
Robert Clark

Collaborators

Ben Moore

Fields of Research

3103 - Ecology;
3108 - Plant biology;
410205 - Fire ecology

Socio-Economic Objective

280102 - Expanding knowledge in the biological sciences;
280111 - Expanding knowledge in the environmental sciences

Keywords

Eucalyptus leaves;
Wildfire;
Diet quality;
Plant secondary metabolites;
Epicormic growth

Type of Research Activity

Pure basic research

Date Coverage

Date To
27/11/2020

Date From
2/11/2021

Date of data creation

2020

Year of data publication

2026

Creator(s) for Citation

Surname
Lane

Given Name
Murraya

Surname
Marsh

Given Name
Karen

Surname
Youngentob

Given Name
Kara

Surname
Skewes

Given Name
James

Publisher for Citation

The Australian National University Data Commons

Access Rights

Open Access allowed

Access Rights Type

Open

Licence Type

CC-BY-NC-ND - Attribution-NonCommercial-NoDervis (Version 3.0)

Data Location

ANU Data Commons

Retention Period

Indefinitely

Extent or Quantity

1

Data Size

101 KB

Data Management Plan

No

[Download data files](https://datacommons.anu.edu.au/DataCommons/rest/records/anudc:6457/data/)

Number of files: 1

Size: 101 KB

Identifier: anudc:6457

Status: Published\
Published to:

- Australian National University
- Australian National Data Service

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- hasAssociationWith:\
  [Karen Marsh](https://datacommons.anu.edu.au/DataCommons/rest/display/anudc:6440) [anudc:6440]
- hasAssociationWith:\
  [Dr. Kara N. Youngentob](https://datacommons.anu.edu.au/DataCommons/rest/display/anudc:6454) [anudc:6454]
- hasAssociationWith:\
  [Associate Prof. Robert Clark](https://datacommons.anu.edu.au/DataCommons/rest/display/anudc:6458) [anudc:6458]
- hasAssociationWith:\
  [Associate Prof. Ben D. Moore](https://datacommons.anu.edu.au/DataCommons/rest/display/anudc:6459) [anudc:6459]
- hasPrincipalInvestigator:\
  [Murraya Lane](https://datacommons.anu.edu.au/DataCommons/rest/display/anudc:6439) [anudc:6439]