Tenascin-c functionalised self-assembling peptide hydrogels for critical-sized bone defect reconstruction
Source: https://eprints.gla.ac.uk/359951/ Parent: https://eprints.gla.ac.uk/view/project_code/315918.html
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Tenascin-c functionalised self-assembling peptide hydrogels for critical-sized bone defect reconstruction
Trubert-Paneli, A. et al. (2026) Tenascin-c functionalised self-assembling peptide hydrogels for critical-sized bone defect reconstruction. Biomaterials, 325, 123553. (doi: 10.1016/j.biomaterials.2025.123553)
| Text 359951.pdf - Published Version Available under License Creative Commons Attribution. 10MB |
Abstract
Critical-sized bone defects are unable to heal spontaneously and receive poor clinical prognosis due to limitations in modern treatment strategies. Next-generation therapies are applying biomaterials incorporating BMP-2 to effectively promote and support bone regeneration, but adverse effects are linked to uncontrolled BMP-2 egress from the biomaterial. Implementing extracellular matrix proteins to biomaterials is a favourable approach to alleviate these drawbacks, and self-assembling peptide hydrogels are rapidly emerging as modulable and versatile biomaterials. Here, we describe the creation of a tenascin-c-functionalised peptide hydrogel designed to regenerate critical-sized bone defects. A recombinant fragment of tenascin-c spanning from the 3rd to 5th fibronectin-like domains is integrated into the fibre network. We demonstrate that this nascent construct effectively retains BMP-2 to differentiate mesenchymal stem cells into mature osteoblasts and achieves complete unionisation of murine critical-sized bone defects under low BMP-2 dose. All in all, we demonstrate tenascin-c as a suitable candidate to functionalise biomaterials intended for bone engineering applications and the promising potential of self-assembling peptide hydrogels in treating critical-sized bone defects.
| Item Type: | Articles |
| Additional Information: | The work was supported by funding from the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 874889 - HEALIKICK), European Research Council AdG (101054728) and EPSRC through the Transformative Healthcare Technologies Programme Grant ‘Mechanomeds’ (EP/X033554/1). C.G-G. acknowledges support from the Engineering and Physical Sciences Research Council (Grant No. EPSRC NIA – EP/T000457/1). A.T.P. acknowledges the companies Manchester BIOGEL Ltd. and Cell Guidance Systems Ltd. for their support. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Salmeron-Sanchez, Professor Manuel and Trubert-Paneli, Dr Alexandre and Pringle, Eonan and Gonzalez Garcia, Dr Cristina |
| Authors: | Trubert-Paneli, A., Williams, J. A., Windmill, J. F. C., Oñarte-Echevarria, L. I., Pringle, E. W., Rogkoti, T., Dong, S., Cipitria, A., Miller, A. F., Gonzalez-Garcia, C., Saiani, A., and Salmeron-Sanchez, M. |
| College/School: | College of Science and Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Biomaterials |
| Publisher: | Elsevier |
| ISSN: | 0142-9612 |
| ISSN (Online): | 1878-5905 |
| Published Online: | 11 July 2025 |
| Copyright Holders: | Copyright © 2025 The Authors |
| First Published: | First published in Biomaterials 325: 123553 |
| Publisher Policy: | Reproduced under a Creative Commons license |
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Funder and Project Information
Funder and Project Information
Funder and Project Information
Project Code
Award No
Project Name
Principal Investigator
Funder's Name
Funder Ref
Lead Dept
HEALIKICK
Manuel Salmeron-Sanchez
874889
School of Molecular Biosciences
DEVISE - Engineered viscoelasticity in regenerative microenvironments
Manuel Salmeron-Sanchez
101054728
ENG - Biomedical Engineering
Mechanobiology-based medicine - Phase 2
Manuel Salmeron-Sanchez
Engineering and Physical Sciences Research Council (EPSRC)
EP/X033554/1
ENG - Biomedical Engineering
Bioactive polysaccharide-based hydrogels for growth factors delivery during tissue repair.
Cristina Gonzalez Garcia
Engineering and Physical Sciences Research Council (EPSRC)
EP/T000457/1
ENG - Biomedical Engineering
Deposit and Record Details
| ID Code: | 359951 |
| Depositing User: | Ms Gail Annan |
| Datestamp: | 15 Jul 2025 11:39 |
| Last Modified: | 23 Jul 2025 09:48 |
| Date of acceptance: | 10 July 2025 |
| Date of first online publication: | 11 July 2025 |
| Date Deposited: | 23 July 2025 |
| Data Availability Statement: | No |
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