Enlighten Theses — Study Abroad Crawler

Metadata
Title: Enlighten Theses
Category: general
UUID: c6105709c9b64c50a7fbac37d97b6276
Source URL: https://theses.gla.ac.uk/9143/
Parent URL: https://theses.gla.ac.uk/view/subjects/QH345.html
Crawl Time: 2026-03-11T07:42:31+00:00
# Enlighten Theses

**Source**: https://theses.gla.ac.uk/9143/
**Parent**: https://theses.gla.ac.uk/view/subjects/QH345.html

- [Skip to main content](#mainpage)
- [Accessibility information](http://www.gla.ac.uk/about/accessibility/)

Site navigation

- [Study](http://www.gla.ac.uk/study/)
- [Research](http://www.gla.ac.uk/research/)
- [About us](http://www.gla.ac.uk/about/)
- [Student life](http://www.gla.ac.uk/studentlife/)
- [Alumni](http://www.gla.ac.uk/alumni/)
- [Support us](http://www.gla.ac.uk/about/givingtoglasgow/)
- [Contact](http://www.gla.ac.uk/about/contact/)

Site tools

A-Z Lists

- [Subjects A-Z](http://www.gla.ac.uk/subjects/)
- [Staff A-Z](http://www.gla.ac.uk/stafflist/)
- [Academic units A-Z](http://www.gla.ac.uk/academic/)

- [Home](http://www.glasgow.ac.uk)
- [Enlighten](http://www.glasgow.ac.uk/services/enlighten)
- [Theses](http://theses.gla.ac.uk)

# [Enlighten Theses](http://theses.gla.ac.uk)

- [About](http://theses.gla.ac.uk/information.html)
- [Latest Additions](https://theses.gla.ac.uk/cgi/latest)
- [Search](https://theses.gla.ac.uk/cgi/search/advanced)
- [Browse](http://theses.gla.ac.uk/view/)
  - [Browse by
    Year](http://theses.gla.ac.uk/view/year/)
  - [Browse by
    Subject](http://theses.gla.ac.uk/view/subjects/)
  - [Browse by
    College/School](http://theses.gla.ac.uk/view/divisions/)
  - [Browse by
    Author](http://theses.gla.ac.uk/view/creators/)
  - [Browse by
    Funder](http://theses.gla.ac.uk/view/funder/)
- [ORCID](https://www.gla.ac.uk/myglasgow/research/managingyourpublications/publicationsandresearchreputation/identifiers/orcid/)
- [My Thesis](#)
  - [Login (Library staff only)](https://theses.gla.ac.uk/cgi/users/home)

In this section

# Engineering chimaeric recombinases for HIV-1 proviral DNA excision

Abioye, Jumai Adeola 
(2018)
*Engineering chimaeric recombinases for HIV-1 proviral DNA excision.*
PhD thesis, University of Glasgow.

Full text available as:

|  |  |
| --- | --- |
|  | PDF   [Download (12MB)](https://theses.gla.ac.uk/9143/1/2018abioyephd.pdf) |

Printed Thesis Information: <https://eleanor.lib.gla.ac.uk/record=b3310662>

## Abstract

‘Cutting out’ HIV-1 proviral DNA could potentially cure a person of the infection. Genome editing approaches have been proffered for eradicating the provirus in infected persons by activating all latent viral reservoirs for further antiretroviral therapy or for the excision of the proviral DNA from memory T- cells. Previous approaches to do this have used nuclease-based tools or reprogrammed tyrosine recombinases; the former presenting unpredictable therapeutic outcomes and the latter, lengthy design time for newer tool variants if viral mutability erodes their effectiveness. Unlike nuclease-based tools that only cut DNA and rely on host-mediated repair mechanisms, chimaeric recombinases (CRs) cut DNA and carry the inherent ability to re-ligate cut ends at the cleavage site. The modular domain architecture of small serine recombinases can be redesigned to mediate site-specific recombination on non-cognate sites, by replacing the C-terminal DNA binding domains (DBDs) of serine recombinases with programmable DBDs such as Zinc Finger (ZF) proteins, TAL effector proteins and CRISPR-dCas9. For HIV-1 proviral DNA excision, CR requirement for the interaction of two recombinase-bound sites, and the lack of necessity for host cell-encoded factors should maximize the fidelity and efficiency of provirus removal.

In this work, the engineering and characterization of CRs with the specificity to recognize and promote site-specific recombination at highly conserved regions within the HIV-1 proviral DNA is explored. This research provides a solid proof-of-concept for the use of CRs to target divergent novel target sequences, expanding their applicability for applied genome editing and wider biotechnological applications.

|  |  |
| --- | --- |
| Item Type: | Thesis (PhD) |
| Qualification Level: | Doctoral |
| Keywords: | Chimaeric recombinase, genome editing, recombination, synthetic biology, protein engineering, bioengineering, molecular biology. |
| Subjects: | [Q Science](https://theses.gla.ac.uk/view/subjects/Q.html) > [QH Natural history](https://theses.gla.ac.uk/view/subjects/QH.html) > [QH345 Biochemistry](https://theses.gla.ac.uk/view/subjects/QH345.html) [Q Science](https://theses.gla.ac.uk/view/subjects/Q.html) > [QH Natural history](https://theses.gla.ac.uk/view/subjects/QH.html) > [QH426 Genetics](https://theses.gla.ac.uk/view/subjects/QH426.html) [Q Science](https://theses.gla.ac.uk/view/subjects/Q.html) > [QR Microbiology](https://theses.gla.ac.uk/view/subjects/QR.html) |
| Colleges/Schools: | [College of Science and Engineering](https://theses.gla.ac.uk/view/divisions/30000000/) > [School of Engineering](https://theses.gla.ac.uk/view/divisions/30300000/) > [Biomedical Engineering](https://theses.gla.ac.uk/view/divisions/30303000/) |
| Funder's Name: | [Engineering and Physical Sciences Research Council (EPSRC)](https://theses.gla.ac.uk/view/funder/Engineering_and_Physical_Sciences_Research_Council_=28EPSRC=29.html) |
| Supervisor's Name: | Cooper, Professor Jonathan M., Reboud, Dr. Julien and Stark, Professor Marshall W. |
| Date of Award: | 2018 |
| Depositing User: | [J.A. Abioye](https://theses.gla.ac.uk/cgi/users/home?screen=User::View&userid=15974) |
| Unique ID: | glathesis:2018-9143 |
| Copyright: | Copyright of this thesis is held by the author. |
| Date Deposited: | 29 May 2018 08:53 |
| Last Modified: | 17 Feb 2023 08:48 |
| Thesis DOI: | [10.5525/gla.thesis.9143](https://doi.org/10.5525/gla.thesis.9143) |
| URI: | <https://theses.gla.ac.uk/id/eprint/9143> |

### Actions (login required)

|  |  |
| --- | --- |
|  | View Item |

### Downloads

Downloads per month over past year

Tools

[Tools](#)

[Tools](#)

RDF+XML
RDF+N-Triples
JSON
RefWorks
Dublin Core
Atom
Simple Metadata
Refer
METS
HTML Citation
ASCII Citation
OpenURL ContextObject
EndNote
OpenURL ContextObject in Span
MODS
MPEG-21 DIDL
EP3 XML
Data Cite XML
Reference Manager
RDF+N3
Multiline CSV

\

[Back to top](#)

## [Library](http://www.gla.ac.uk/services/library/)

- [Contact us](http://www.gla.ac.uk/services/library/contact/)

The University of Glasgow is a registered Scottish charity: Registration Number SC004401