NaBC1 boron transporter enables myoblast response to substrate rigidity via fibronectin-binding integrins
Source: https://eprints.gla.ac.uk/330220/ Parent: https://eprints.gla.ac.uk/view/project_code/315918.html
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NaBC1 boron transporter enables myoblast response to substrate rigidity via fibronectin-binding integrins
Gonzalez-Valdivieso, Juan, Ciccone, Giuseppe, Dhawan, Udesh ORCID: https://orcid.org/0000-0002-0393-9414, Quon, Tezz, Barcelona-Estaje, Eva, Rodrigo-Navarro, Aleixandre ORCID: https://orcid.org/0000-0002-3786-0464, Castillo, Rafael R., Milligan, Graeme ORCID: https://orcid.org/0000-0002-6946-3519, Rico, Patricia and Salmeron-Sanchez, Manuel ORCID: https://orcid.org/0000-0002-8112-2100 (2025) NaBC1 boron transporter enables myoblast response to substrate rigidity via fibronectin-binding integrins. Advanced Science, 12(20), 2407548. (doi: 10.1002/advs.202407548) (PMID:40270477) (PMCID:PMC12120709)
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Abstract
Cells are sensitive to the physical properties of their microenvironment and transduce them into biochemical cues that trigger gene expression and alter cell behavior. Numerous proteins, including integrins, are involved in these mechanotransductive events. Here, a novel role for the boron transporter NaBC1 is identified as a mechanotransducer. It is demonstrated that soluble boron ions activate NaBC1 to enhance cell adhesion and intracellular tension in C2C12 myoblasts seeded on fibronectin-functionalized polyacrylamide (PAAm) hydrogels. Retrograde actin flow and traction forces exerted by these cells are significantly increased in vitro in response to both increased boron concentration and hydrogel stiffness. These effects are fibronectin and NaBC1-mediated as they are abrogated in hydrogels coated with laminin-111 in place of fibronectin and in esiRNA NaBC1-silenced cells. These findings thus demonstrate that NaBC1 controls boron homeostasis and also functions as a mechanosensor.
| Item Type: | Articles |
| Additional Information: | M.S-S is grateful for financial support from the European Research Council AdG (Devise, 101054728) and EPSRC HT2050 grant (EP/X033554/1). P.R acknowledges support by grant PID2021-126012OBI00 funded by MCIN/AEI/10.13039/501100011033 and by ERDF a way of making Europe, and by CIBER (CB06/01/1026). J.G-V acknowledges the funding from the European Union Next Generation EU program. |
| Keywords: | Mechanobiology, tissue engineering, biomaterials, muscle cells, NaBC1. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Salmeron-Sanchez, Professor Manuel and Quon, Dr Tezz and Milligan, Professor Graeme and CICCONE, Mr GIUSEPPE and Rodrigo-Navarro, Mr Aleixandre and Dhawan, Dr Udesh |
| Creator Roles: | CICCONE, G.Methodology, Investigation, Writing – review and editing Salmeron-Sanchez, M.Conceptualization, Writing – review and editing, Funding acquisition, Resources, Supervision |
| Authors: | Gonzalez-Valdivieso, J., Ciccone, G., Dhawan, U., Quon, T., Barcelona-Estaje, E., Rodrigo-Navarro, A., Castillo, R. R., Milligan, G., Rico, P., and Salmeron-Sanchez, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Advanced Science |
| Publisher: | Wiley |
| ISSN: | 2198-3844 |
| ISSN (Online): | 2198-3844 |
| Published Online: | 24 April 2025 |
| Copyright Holders: | Copyright © 2025 The Author(s) |
| First Published: | First published in Advanced Science 12(20):e2407548 |
| Publisher Policy: | Reproduced under a Creative Commons license |
| Related URLs: | - Pre-print |
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Funder and Project Information
Funder and Project Information
Funder and Project Information
Project Code
Award No
Project Name
Principal Investigator
Funder's Name
Funder Ref
Lead Dept
DEVISE - Engineered viscoelasticity in regenerative microenvironments
Manuel Salmeron-Sanchez
101054728
ENG - Biomedical Engineering
Mechanobiology-based medicine - Phase 2
Manuel Salmeron-Sanchez
Engineering and Physical Sciences Research Council (EPSRC)
EP/X033554/1
ENG - Biomedical Engineering
Deposit and Record Details
| ID Code: | 330220 |
| Depositing User: | Mr Alastair Arthur |
| Datestamp: | 19 Jul 2024 13:52 |
| Last Modified: | 11 Jun 2025 08:38 |
| Date of acceptance: | 2 April 2025 |
| Date of first online publication: | 24 April 2025 |
| Date Deposited: | 2 April 2025 |
| Data Availability Statement: | Yes |
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