# 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation
**Source**: https://eprints.gla.ac.uk/183673/
**Parent**: https://eprints.gla.ac.uk/view/project_code/303613.html
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# 3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation
Re, F. et al.
(2019)
3D gelatin-chitosan hybrid hydrogels combined with human platelet lysate highly support human mesenchymal stem cell proliferation and osteogenic differentiation.
*[Journal of Tissue Engineering](https://eprints.gla.ac.uk/view/journal_volume/Journal_of_Tissue_Engineering.html)*, 10,
pp. 1-16.
(doi: [10.1177/2041731419845852](https://doi.org/10.1177/2041731419845852))
(PMID:[31105928](https://europepmc.org/abstract/MED/31105928))
(PMCID:[PMC6507314](https://europepmc.org/articles/PMC6507314))
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| Preview | [Text](https://eprints.gla.ac.uk/183673/7/183673.pdf) 183673.pdf - Published Version Available under License [Creative Commons Attribution Non-commercial](http://creativecommons.org/licenses/by-nc/4.0/). 1MB |
## Abstract
Bone marrow and adipose tissue human mesenchymal stem cells were seeded in highly performing 3D gelatin–chitosan hybrid hydrogels of varying chitosan content in the presence of human platelet lysate and evaluated for their proliferation and osteogenic differentiation. Both bone marrow and adipose tissue human mesenchymal stem cells in gelatin–chitosan hybrid hydrogel 1 (chitosan content 8.1%) or gelatin–chitosan hybrid hydrogel 2 (chitosan 14.9%) showed high levels of viability (80%–90%), and their proliferation and osteogenic differentiation was significantly higher with human platelet lysate compared to fetal bovine serum, particularly in gelatin–chitosan hybrid hydrogel 1. Mineralization was detected early, after 21 days of culture, when human platelet lysate was used in the presence of osteogenic stimuli. Proteomic characterization of human platelet lysate highlighted 59 proteins mainly involved in functions related to cell adhesion, cellular repairing mechanisms, and regulation of cell differentiation. In conclusion, the combination of our gelatin–chitosan hybrid hydrogels with hPL represents a promising strategy for bone regenerative medicine using human mesenchymal stem cells.
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| Item Type: | Articles |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | [Re, Federica](https://eprints.gla.ac.uk/view/author/43217.html) and [Cantini, Dr Marco](https://eprints.gla.ac.uk/view/author/30437.html) and [Salmeron-Sanchez, Professor Manuel](https://eprints.gla.ac.uk/view/author/30067.html) |
| Authors: | [Re, F.](https://eprints.gla.ac.uk/view/author/43217.html), [Cantini, M.](https://eprints.gla.ac.uk/view/author/30437.html), Almici, C., Bianchetti, A., Chinello, C., Dey, K., Agnelli, S., Manferdini, C., Bernardi, S., Lopomo, N. F., Sardini, E., Borsani, E., Rodella, L. F., Savoldi, F., Paganelli, C., Guizzi, P., Lisignoli, G., Magni, F., [Salmeron-Sanchez, M.](https://eprints.gla.ac.uk/view/author/30067.html), and Russo, D. |
| College/School: | [College of Science and Engineering](https://eprints.gla.ac.uk/view/divisions/30000000/) [College of Science and Engineering](https://eprints.gla.ac.uk/view/divisions/30000000/) > [School of Engineering](https://eprints.gla.ac.uk/view/divisions/30300000/) > [Biomedical Engineering](https://eprints.gla.ac.uk/view/divisions/30303000/) |
| Journal Name: | [Journal of Tissue Engineering](https://eprints.gla.ac.uk/view/journal_volume/Journal_of_Tissue_Engineering.html) |
| Publisher: | SAGE |
| ISSN: | 2041-7314 |
| ISSN (Online): | 2041-7314 |
| Published Online: | 02 May 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Journal of Tissue Engineering 10:1-16 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: [Request a correction](https://eprints.gla.ac.uk/cgi/request_correction?eprintid=183673) | Enlighten Editors: [Update this record](https://eprints.gla.ac.uk/cgi/users/home?screen=EPrint::View&eprintid=183673)
Funder and Project Information
[Funder and Project Information](#)
[Funder and Project Information](#)
Project Code
Award No
Project Name
Principal Investigator
Funder's Name
Funder Ref
Lead Dept
[72206](https://eprints.gla.ac.uk/view/project_code/72206.html)
1
Engineering growth factor microenvironments- a new therapeutic paradigm for regenerative medicine
Manuel Salmeron-Sanchez
[Engineering and Physical Sciences Research Council (EPSRC)](https://eprints.gla.ac.uk/view/funder/Engineering_and_Physical_Sciences_Research_Council_=28EPSRC=29.html)
EP/P001114/1
ENG - BIOMEDICAL ENGINEERING
[303613](https://eprints.gla.ac.uk/view/project_code/303613.html)
0
Engineered microenvironments to harvest stem cell response to viscosity for cartilage repair
Marco Cantini
[Medical Research Council (MRC)](https://eprints.gla.ac.uk/view/funder/Medical_Research_Council_=28MRC=29.html)
MR/S005412/1
ENG - Biomedical Engineering
Deposit and Record Details
[Deposit and Record Details](#)
[Deposit and Record Details](#)
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| ID Code: | 183673 |
| Depositing User: | [Dr Aniko Szilagyi](https://eprints.gla.ac.uk/profile/16852) |
| Datestamp: | 08 Apr 2019 14:40 |
| Last Modified: | 02 May 2025 05:57 |
| Date of acceptance: | 2 April 2019 |
| Date of first online publication: | 2 May 2019 |
| Date Deposited: | 9 April 2019 |
| Data Availability Statement: | No |
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