Metadata
Title
Yale University
Category
general
UUID
07fa51e243c54bd6a1cc7d266cfb9016
Source URL
https://cbic.yale.edu/instruments-listing
Parent URL
https://cbic.yale.edu/instruments
Crawl Time
2026-03-10T04:39:13+00:00
Rendered Raw Markdown

Yale University

Source: https://cbic.yale.edu/instruments-listing Parent: https://cbic.yale.edu/instruments

Instrument Catalog

Instrument Type

Instrument Location

Sort by Instrument NameDate of AcquisitionInstrument Type

Order AscDesc

**Chemical Crystallography User Policy**

Short Name: Small Molecule Diffraction Policy

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: September 2013

\

Co-authorship suggestions

Please note that single crystal diffraction/model prices do not fluctuate based on effort or time and are not waived even if the diffraction model warrants co-authorship. Should you publish data collected in our center, please acknowledge our facility in your manuscripts or grant applications as follows:

This research made use of the Chemical and Biophysical Instrumentation Center at Yale University (RRID:SCR_021738).

Dr. Mercado is available to assist with the diffraction components of experiments and review manuscripts. Co-authorship should be considered if:

  1. The structural information in the report is derived primarily from diffraction data and constitutes a significant part of the writing.
  2. The structure determination is non-routine, such as involving variable temperature studies, phase changes, or specialized collection environments.
  3. The final structure required a substantial amount of time and technical expertise. For instance:
  4. Problematic twinning or significant disorder in structure refinement.
  5. Careful consideration of anomalous dispersion (e.g., absolute stereochemistry).
  6. Determination of the model by MicroED.

Center Use Policy

**Chemical Crystallography User Policy**

Short Name: Small Molecule Diffraction Policy

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: September 2013

\

Co-authorship suggestions

Please note that single crystal diffraction/model prices do not fluctuate based on effort or time and are not waived even if the diffraction model warrants co-authorship. Should you publish data collected in our center, please acknowledge our facility in your manuscripts or grant applications as follows:

This research made use of the Chemical and Biophysical Instrumentation Center at Yale University (RRID:SCR_021738).

Dr. Mercado is available to assist with the diffraction components of experiments and review manuscripts. Co-authorship should be considered if:

  1. The structural information in the report is derived primarily from diffraction data and constitutes a significant part of the writing.
  2. The structure determination is non-routine, such as involving variable temperature studies, phase changes, or specialized collection environments.
  3. The final structure required a substantial amount of time and technical expertise. For instance:
  4. Problematic twinning or significant disorder in structure refinement.
  5. Careful consideration of anomalous dispersion (e.g., absolute stereochemistry).
  6. Determination of the model by MicroED.

Center Use Policy

500wb Bruker Neo 500 MHz NMR spectrometer

Short Name: V500wb

Instrument Type: NMR & EPR

Instrument Location: CRB 100

Date of Acquisition: December 1995

\

500 MHz wide-bore NMR spectrometer.

3.2 mm CPMAS probe normally installed for solid-state NMR experiments.

Micro MRI imaging capabilities

A400a Agilent DD2 400 MHz NMR Spectrometer (Autosampler)

Short Name: A400a

Instrument Type: NMR & EPR

Instrument Location: KCL 3

Date of Acquisition: August 2013

\

2 channel system with AS7620 96-sample changer. OneNMR\ probe with auto-tune-and-match installed.

Undergraduates have priority on this instrument during lab periods.

Guides and Standard Operating Procedures

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A400a:
Mass Concentration Proton Carbon gCOSY gHSQC gHMBC
0.03 mg 0.1 mM 10 h 40 min 35389 hours 1785 hours 1886 hours 7129 hours
0.06 mg 0.2 mM 2 h 40 min 10516 hours 46 h 28 min 474 h 12 min 1785 hours
0.15 mg 0.5 mM 0 h 26 min 1415 h 33 min 6 h 10 min 75 h 01 min 284 h 44 min
0.3 mg 1 mM 0 h 06 min 354 h 01 min 1 h 40 min 18 h 53 min 71 h 19 min
0.6 mg 2 mM 0 h 01 min 88 h 31 min 0 h 26 min 4 h 42 min 17 h 51 min
1.5 mg 5 mM < 1 min 14 h 09 min 0 h 03 min 0 h 45 min 2 h 51 min
3 mg 10 mM < 1 min 3 h 32 min 0 h 03 min 0 h 16 min 1 h 07 min
6 mg 20 mM < 1 min 0 h 53 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 13 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 08 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A400b Agilent DD2 400 MHz NMR Spectrometer (Manual)

Short Name: A400b

Instrument Type: NMR & EPR

Instrument Location: KCL 3

Date of Acquisition: October 2013

\

2 channel system. AutoX DB probe installed.  Variable-temperature and H/F experiments available.

Guides and Standard Operating Procedures

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A400b:
Mass Concentration Proton Carbon gCOSY gHSQC gHMBC
0.03 mg 0.1 mM 44 h 44 min 50960 hours 644 hours 7862 hours 29844 hours
0.06 mg 0.2 mM 11 h 11 min 12740 hours 161 hours 1965 hours 7461 hours
0.15 mg 0.5 mM 1 h 47 min 2038 h 24 min 25 h 47 min 319 h 18 min 1193 h 57 min
0.3 mg 1 mM 0 h 27 min 509 h 36 min 6 h 30 min 78 h 37 min 298 h 40 min
0.6 mg 2 mM 0 h 07 min 127 h 27 min 1 h 38 min 19 h 48 min 75 h 40 min
1.5 mg 5 mM 0 h 01 min 20 h 23 min 0 h 16 min 3 h 08 min 12 h 15 min
3 mg 10 mM < 1 min 5 h 15 min 0 h 06 min 0 h 47 min 1 h 32 min
6 mg 20 mM < 1 min 1 h 16 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 19 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 12 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A400c Agilent DD2 400 MHz NMR Spectrometer (Autosampler)

Short Name: A400c

Instrument Type: NMR & EPR

Instrument Location: KCL 3

Date of Acquisition: October 2013

\

2 channel system with AS7620 96-sample changer. OneNMR probe with auto-tune-and-match installed.

Primarily used for short, routine NMR experiments where data is needed quickly.

Guides and Standard Operating Procedures

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A400c:
Mass Concentration Proton Carbon gCOSY gHSQC gHMBC
0.03 mg 0.1 mM 10 h 40 min 35389 hours 1785 hours 1886 hours 7129 hours
0.06 mg 0.2 mM 2 h 40 min 10516 hours 46 h 28 min 474 h 12 min 1785 hours
0.15 mg 0.5 mM 0 h 26 min 1415 h 33 min 6 h 10 min 75 h 01 min 284 h 44 min
0.3 mg 1 mM 0 h 06 min 354 h 01 min 1 h 40 min 18 h 53 min 71 h 19 min
0.6 mg 2 mM 0 h 01 min 88 h 31 min 0 h 26 min 4 h 42 min 17 h 51 min
1.5 mg 5 mM < 1 min 14 h 09 min 0 h 03 min 0 h 45 min 2 h 51 min
3 mg 10 mM < 1 min 3 h 32 min 0 h 03 min 0 h 16 min 1 h 07 min
6 mg 20 mM < 1 min 0 h 53 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 13 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 08 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A500a Agilent DD2 500 MHz NMR Spectrometer (Manual)

Short Name: A500a

Instrument Type: NMR & EPR

Instrument Location: KCL 3

Date of Acquisition: January 2014

\

2 channel system, OneNMR probe with auto-tune-and-match installed.

Guides and Standard Operating Procedures

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A500a:
Mass Concentration Proton Carbon gCOSY gHSQC gHMBC
0.03 mg 0.1 mM 5 hours 16962 hours 67 h 16 min 820 h 42 min 3113 h 37 min
0.06 mg 0.2 mM 1 h 10 min 424 h 02 min 16 h 50 min 205 h 15 min 779 h 06 min
0.15 mg 0.5 mM 0 h 11 min 678 h 28 min 2 h 45 min 32 h 50 min 124 h 34 min
0.3 mg 1 mM 0 h 03 min 170 h 00 min 0 h 42 min 8 h 20 min 31 h 09 min
0.6 mg 2 mM < 1 min 4 h 15 min 0 h 11 min 2 h 05 min 7 h 48 min
1.5 mg 5 mM < 1 min 3 h 11 min 0 h 03 min 0 h 08 min 1 h 40 min
3 mg 10 mM < 1 min 1 h 42 min 0 h 03 min 0 h 08 min 0 h 17 min
6 mg 20 mM < 1 min 0 h 25 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 06 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A500b Agilent DD2 500 MHz NMR Spectrometer (Manual)

Short Name: A500b

Instrument Type: NMR & EPR

Instrument Location: KCL 3

Date of Acquisition: September 2012

\

2 channel system, OneNMR probe with auto-tune-and-match installed.

Guides and Standard Operating Procedures

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A500b:
Mass Concentration Proton Carbon gCOSY gHSQC gHMBC
0.03 mg 0.1 mM 5 hours 16962 hours 67 h 16 min 820 h 42 min 3113 h 37 min
0.06 mg 0.2 mM 1 h 10 min 424 h 02 min 16 h 50 min 205 h 15 min 779 h 06 min
0.15 mg 0.5 mM 0 h 11 min 678 h 28 min 2 h 45 min 32 h 50 min 124 h 34 min
0.3 mg 1 mM 0 h 03 min 170 h 00 min 0 h 42 min 8 h 20 min 31 h 09 min
0.6 mg 2 mM < 1 min 4 h 15 min 0 h 11 min 2 h 05 min 7 h 48 min
1.5 mg 5 mM < 1 min 3 h 11 min 0 h 03 min 0 h 08 min 1 h 40 min
3 mg 10 mM < 1 min 1 h 42 min 0 h 03 min 0 h 08 min 0 h 17 min
6 mg 20 mM < 1 min 0 h 25 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 06 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A600a Agilent DD2 600 MHz NMR Spectrometer (Autosampler)

Short Name: A600a

Instrument Type: NMR & EPR

Instrument Location: KCL 3

Date of Acquisition: October 2013

\

3 channel system with AS7510 12-sample automatic sample changer. C{H} cold probe with auto-tune-and-match installed. Highest sensitivity for C13 NMR experiments.

Guides and Standard Operating Procedures

Best experiments:

Acceptable experiments:

Not available:

Time Required for NMR Experiments on A600a:
Mass Concentration Proton Carbon gCOSY gHSQC gHMBC
0.03 mg 0.1 mM 0 h 24 min 1298 hours 5 h 54 min 72 h 04 min 272 h 22 min
0.06 mg 0.2 mM 0 h 06 min 324 h 32 min 1 h 28 min 19 h 35 min 68 h 32 min
0.15 mg 0.5 mM 0 h 01 min 51 h 56 min 0 h 16 min 3 h 11 min 11 h 10 min
0.3 mg 1 mM < 1 min 13 h 00 min 0 h 03 min 0 h 47 min 2 h 47 min
0.6 mg 2 mM < 1 min 3 h 15 min 0 h 03 min 0 h 16 min 1 h 07 min
1.5 mg 5 mM < 1 min 0 h 31 min 0 h 03 min 0 h 08 min 0 h 34 min
3 mg 10 mM < 1 min 0 h 08 min 0 h 03 min 0 h 08 min 0 h 17 min
6 mg 20 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
12 mg 40 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
15 mg 50 mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min
> 30 mg 100+ mM < 1 min 0 h 04 min 0 h 03 min 0 h 08 min 0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A800 Agilent 800 MHz NMR Spectrometer (Manual)

Short Name: A800

Instrument Type: NMR & EPR

Instrument Location: CRB 100

Date of Acquisition: July 2013

\

4 channel system. Inverse-detect HCN probe, Dual Band (direct-detect) probe, and H{CN} C13-sensitivity enhanced salt-tolerant cold probe available.

Experienced Users Only. Contact CBIC staff for more information.

Analytical Ultracentrifuge Beckman Coulter Proteomelab XL-I

Short Name: AU

Instrument Type: Solution Biophysics

Instrument Location: KCL 3

Date of Acquisition: November 2009

\

Analytical ultracentrifugation is a column-free separation technique that measures the relative change in the distribution of molecular weights, providing a way to measure heterogeneity, stoichiometry and self-association of proteins, oligomers, aggregates, particles, colloids, and small structures in solution.

Applied Photophysics Chirascan Circular Dichroism Spectrometer

Short Name: CD

Instrument Type: Solution Biophysics

Instrument Location: KCL 3

Date of Acquisition: September 2013

\

Chirascan is a modern, high performance circular dichroism (CD) spectrometer operating in the ultraviolet and visible wavelength range. It is typically used to probe the secondary structure or conformation of bio-molecules.

Standard Operating Procedure

DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II

Short Name: DLS

Instrument Type: Scattering, Instrument Type: Solution Biophysics, Instrument Type: Structural Science

Instrument Location: KCL 3

Date of Acquisition: October 2013

\

Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.

Typical applications for DLS include:

The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.

Standard Operating Procedure

DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II

Short Name: DLS

Instrument Type: Scattering, Instrument Type: Solution Biophysics, Instrument Type: Structural Science

Instrument Location: KCL 3

Date of Acquisition: October 2013

\

Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.

Typical applications for DLS include:

The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.

Standard Operating Procedure

DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II

Short Name: DLS

Instrument Type: Scattering, Instrument Type: Solution Biophysics, Instrument Type: Structural Science

Instrument Location: KCL 3

Date of Acquisition: October 2013

\

Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.

Typical applications for DLS include:

The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.

Standard Operating Procedure

EPR Bruker EleXsys EPR Spectrometer

Short Name: EPR

Instrument Type: NMR & EPR

Instrument Location: KCL 12

Date of Acquisition: September 2000

\

X-Band Electron Paramagnetic Resonance. Equipped with a cryogen-free cryostat.

FTIR ASI React IR Reaction Monitoring System

Short Name: ReactIR

Instrument Type: Optical Spectroscopy

Instrument Location: KCL 3

Date of Acquisition: June 1998

\

React IR 15 FTIR for real-time monitoring of chemical reactions in situ.

FTIR Shimadzu IRTracer-100

Short Name: IR Tracer

Instrument Type: Optical Spectroscopy

Instrument Location: KCL 3

Date of Acquisition: February 2020

\

The IRTracer-100 offers exceptionally high sensitivity with an SN ratio of 60,000:1, high resolution down to 0.25 cm−1, and fast measurement speeds up to 20 scans per second.\ The system supports a wide variety of applications with features such as a 12,000-entry library and a data analysis program for contaminant analysis and time course and rapid scan functions for tracking reactions.

Available Accessories:

For more compatible accessories for your measurement needs, please have a look at the Shimadzu Selection Guide.

FTIR/Raman Thermo Nicolet 6700

Short Name: FTIR

Instrument Type: Optical Spectroscopy

Instrument Location: KCL 3

Date of Acquisition: November 2006

\

Diamond ATR cell normally installed. Transmission, DRIFTS, ZnSe ATR, and high-temperature accessories available. Raman with IR-laser source also available.

Standard Operating Procedure

GC-MS Agilent 6890N/5973

Short Name: GC-MS

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: February 2004

\

Open access GC-MS instrument with 100-position sample changer and 20 meter column installed.

Standard Operating Procedure

GC-MS column selection

Short Name: GC-MS columns

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: September 2021

\
Column Name Manufacturer Thickness (um) Length (m) Diameter (mm) Max. Temp. (deg C)
SH-Rxi-5Sil MS Shimadzu 0.25 30 0.25 350
DB select 624UI Agilent 1.4 30 0.25 260
ZB-WAX Zebron 0.5 30 0.25 250
PoraPLOT Q Agilent 8 25 0.25 250
EC-1 Grace 0.25 30 0.25 350
VF-WAXms Agilent 1 30 0.25 260
Rtx-1 Restek 0.25 15 0.25 350
Stabilwax-MS Restek 0.25 30 0.25 260

GC-TQ-MS Shimadzu 8050 NX

Short Name: GC-TQ-MS

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: December 2019

\

Shimadzu 8050 NX Triple Quadrupole (TQ) GC-MS with Liquid Injection, Solid Phase Micro Extraction (SPME) and Headspace Analysis Autosampler. Triple Quadrupole MS instruments allow for quantitation of compounds, collision induced dissociation (CID) and multiple reaction monitoring (MRM). The system is equipped with an Electron Ionization (EI) source, a Chemical Ionization (CI) source and a Flame Ionization Detector (FID).

LC-MS Shimadzu 8060 TQ

Short Name: 8060

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: January 2020

\

Service only Shimadzu LCMS-8060 Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS). Triple Quadrupole MS instruments allow for quantitation of compounds, collision induced dissociation (CID) and multiple reaction monitoring (MRM) with high sensitivity. The UPLC system includs a fluidic system with two different solvents, three trays for up to 54 samples each (in GC vials), three UPLC columns (see list below) and a photo-diode array (UV/Vis) detector. 96 and 384 well plates can be used for screening projects, please reach out first.

Column 1: Shim-pack Scepter C18-120 1.9mum - 2.1 x 50mm\ Column 2: Shim-pack Scepter C18-120 1.9mum - 2.1 x 100mm\ Column 3: Shim-pack Scepter C8-120 1.9mum - 2.1 x 100mm

Different columns can be installed on demand.

Standard Solvents available for LC separation:

Water (with 0.1% Formic Acid or 10mM NH4Ac)\ Methanol (with and w/o 0.1% Formic Acid or 10mM NH4Ac)\ Acetonitrile (with and w/o 0.1% Formic Acid or 10mM NH4Ac)

Different solvents can be used on demand.

LC-MS Shimadzu 9030 Quadrupole Time-of-Flight MS

Short Name: 9030

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: January 2020

\

Shimadzu LCMS-9030 Quadrupole Time-of-Flight High-Performance Liquid Chromatograph Mass Spectrometer for high-resolution MS.

Service only high resolution Q-tof MS instrument with an UPLC system, including a fluidic system with two different solvents, three trays for up to 54 samples each (in GC vials), three UPLC columns (see list below) and a photo-diode array (UV/Vis) detector. 96 and 384 well plates can be used for screening projects, please reach out first.

Column 1: Shim-pack Scepter C18-120 1.9mum - 2.1 x 50mm\ Column 2: Shim-pack Scepter C18-120 1.9mum - 2.1 x 100mm\ Column 3: Shim-pack Scepter C8-120 1.9mum - 2.1 x 100mm

Different columns can be installed on demand.

Standard Solvents available for LC separation:

Different solvents can be used on demand.

LC-MS ThermoScientific QExactive Orbitrap (+ IR spectroscopy)

Short Name: Orbi

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: August 2019

\

Service only high resolution QExactive Orbitrap FT-MS instrument with an Dionex 3000 UPLC system, including a fluidic system with up to four different solvents, three trays for up to 40 samples each (in 2 mL GC vials), one UPLC column, a UV/Vis photo-diode array (PDA) and a fluorescence (FL) detector.

The structural mass spectrometry extension on this instrument allows for recording of infrared spectra of mass selected ions in the gas phase. Read more about the technique .

Software for opening and converting .raw files: http://msfragger.nesvilab.org/tutorial_convert.html

LC-MS Waters Xevo Qtof high-resolution MS

Short Name: Xevo

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: November 2018

\

Service only high resolution Q-tof MS instrument with an UPLC system, including a fluidic system with up to four different solvents, two trays for up to 48 samples each (in GC vials), four UPLC columns (see list below) and a photo-diode array (UV/Vis).

Column 1: Acquity UPLC BEH C18 1.7mum - 2.1 x 50mm\ Column 2: Acquity UPLC BEH Glycan 1.7mum - 2.1 x 100mm\ Column 3: Acquity UPLC BEH300 C18 1.7mum - 2.1 x 100mm\ Column 4: Acquity UPLC BEH C8 1.7mum - 2.1 x 100mm

MALDI-ToF-MS Shimadzu AXIMA Confidence

Short Name: MALDI

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: January 2020

\

AXIMA Confidence Linear/Reflectron MALDI-TOF Mass Spectrometer from Shimadzu.

Target Plates available:\ AXIMA 384 well (2.8mm I.D.) plate - P/N: DE1580TA
Number of wells: Sample = 384 Calibrant = 0
Internal diameter of wells: Sample = 2.8mm Calibrant = N/A

FlexiMass-SR48 steel targets - P/N: TO-431R00
Number of wells: Sample = 48 Calibrant = 3
Internal diameter of wells: Sample = 2.8mm Calibrant = 2.2mm

FlexiMass-DS disposable targets - P/N: TO-430R00
Number of wells: Sample = 48 Calibrant = 3
Internal diameter of wells: Sample = 2.8mm Calibrant = 1.5mm

Mass Spectrometry meets Infrared Spectroscopy

Short Name: Mass Spec based CIVP IR Spectroscopy

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: December 2021

\

Mass Spectrometry meets Infrared Spectroscopy

Structural insights into cold, mass selected ions

https://cbic.yale.edu/mass-spectrometry-meets-infrared-spectroscopy

MS recommendations, method descriptions and software

Short Name: MS resources

Instrument Type: Mass Spectrometry

Instrument Location: KCL 3

Date of Acquisition: February 2021

\

Disclaimer: All information on this site is subject to change and the actual experimental methods used might differ from the ones found here!

Recommendations:

MS_Sample_Method_Recommendations_v2.docx

Method Descriptions (methods might have changed over time, please double-check!):

Shimadzu 9030 HRMS Description

Thermo Fisher QExactive Orbitrap HRMS Description

Software:

Data exchange

MS data can be exported as mzXML or mzML files and shared via box or google drive upon request. The files contain TIC and MS data, EIC can be extracted through user analysis. These data files can be opened with MestreNova (updated and fully installed licenses necessary!):

Download the software and license bundle from the CBIC homepage:

https://cbic.yale.edu/yale-only/mnova-download

Close MestreNova (for update of existing installation), and run as Administrator by:

Windows Start menu -> MestreNova Folder -> Right Click on MestreNova -> more -> Run as Administrator

Install the Licenses again, close the software and start it as a normal user.

Complex data workups and simple txt data can be provided based on the project.

Software for opening and converting ThermoFisher .raw files: http://msfragger.nesvilab.org/tutorial_convert.html

Acknowledgements:

If you use CBIC instruments in your research, we request that you acknowledge our facility in your written publications or grant applications as follows:

Acknowledgement: This research made use of the Chemical and Biophysical Instrumentation Center at Yale University (RRID:SCR_021738). Equipment was purchased with funds from Yale University.\ Optional for Orbitrap QExactive MS: NSF MRI grant CHE-1828190.\

Pilatus3R HPC on a 007 VHF+Arc)Sec X-ray Source

Short Name: 007c

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: October 2016

\

The Pilatus3R 200KA detector on our Rigaku 007 HF+ rotating anode with VHF Arc)Sec optics is suitable for solving small molecules samples. This instrument is open to usage by students and staff. λ = 0.7107 Å

Pilatus3R HPC on a 007 VHF+Arc)Sec X-ray Source

Short Name: 007c

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: October 2016

\

The Pilatus3R 200KA detector on our Rigaku 007 HF+ rotating anode with VHF Arc)Sec optics is suitable for solving small molecules samples. This instrument is open to usage by students and staff. λ = 0.7107 Å

Polarimeter Perkin Elmer 341

Short Name: Polarimeter

Instrument Type: Optical Spectroscopy

Instrument Location: KCL 16B

Date of Acquisition: January 1997

\

General-purpose precision Polarimeter with Na and Hg source lamps and Glan-Taylor polarizer.

Standard Operating Procedure

Polarimeter Rudolph Autopol IV

Short Name: Autopol

Instrument Type: Optical Spectroscopy

Instrument Location: KCL 16B

Date of Acquisition: February 2018

\

The AUTOPOL® IV is a microprocessor based automatic polarimeter that is available in one, two and six wavelength versions. The AUTOPOL® IV’s features include: readout in Specific Rotation, Optical Rotation, and concentration, customer defined and labeled programs for push button readout, large LCD display and touchscreen user interface, temperature display and correction, push button wavelength selection, statistics, 3 USB ports, 1-USB 3.0, RS 232 and parallel printer ports.

Rigaku CCD on 007HF+ X-ray Source

Short Name: 007a

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: October 2009

\

The hybrid pixel array detector on our Rigaku 007 HF+ rotating anode X-ray source with HF optics is suitable for solving small-molecule and protein structures by single-crystal X-ray diffraction.  This instrument can also be used to screen crystals before sending them to an x-ray beam line at a synchrotron. λ = 1.5418 Å

Rigaku CCD on 007HF+ X-ray Source

Short Name: 007a

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: October 2009

\

The hybrid pixel array detector on our Rigaku 007 HF+ rotating anode X-ray source with HF optics is suitable for solving small-molecule and protein structures by single-crystal X-ray diffraction.  This instrument can also be used to screen crystals before sending them to an x-ray beam line at a synchrotron. λ = 1.5418 Å

Rigaku CCD on 007HF+Arc)Sec X-ray Source

Short Name: 007b

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: June 2016

\

The Rigaku Saturn 944HG CCD detector on our Rigaku 007 HF+ rotating anode X-ray source with HF Arc)Sec optics is suitable for solving small molecules samples. This instrument is run by the CBIC Staff. λ = 1.5418 Å

Rigaku CCD on 007HF+Arc)Sec X-ray Source

Short Name: 007b

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: June 2016

\

The Rigaku Saturn 944HG CCD detector on our Rigaku 007 HF+ rotating anode X-ray source with HF Arc)Sec optics is suitable for solving small molecules samples. This instrument is run by the CBIC Staff. λ = 1.5418 Å

Rigaku MiniFlex600 Powder X-ray Diffractometer

Short Name: PXRD

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 11

Date of Acquisition: December 2004

\

This powder diffractometer can characterize crystallinity, crystal phases, and, in many cases, the identity of solid samples. Our instrument has a sealed-tube copper X-ray source, a scintillation counter with high dynamic range, and Bragg-Brentano geometry with slits providing high resolution for flat powder samples. λ = 1.5418 Å

Rigaku MiniFlex600 Powder X-ray Diffractometer

Short Name: PXRD

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 11

Date of Acquisition: December 2004

\

This powder diffractometer can characterize crystallinity, crystal phases, and, in many cases, the identity of solid samples. Our instrument has a sealed-tube copper X-ray source, a scintillation counter with high dynamic range, and Bragg-Brentano geometry with slits providing high resolution for flat powder samples. λ = 1.5418 Å

Rigaku XtaLAB Mini II with a hybrid pixel detector

Short Name: mini2

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 11

Date of Acquisition: May 2022

\

The XtaLabMini II is a fully functional benchtop single-crystal X-ray diffractometer with a CCD detector. λ = 0.7107 Å

Rigaku XtaLAB Mini II with a hybrid pixel detector

Short Name: mini2

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 11

Date of Acquisition: May 2022

\

The XtaLabMini II is a fully functional benchtop single-crystal X-ray diffractometer with a CCD detector. λ = 0.7107 Å

Rigaku XtaLAB Synergy, Dualflex, HyPix-Arc 100

Short Name: syn

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 11

Date of Acquisition: January 2023

\

Copper and silver X-ray sources are available. The curved image plate detector allows high dynamic range and collection out to a 2Θ angle of 144 degrees in a single image to yield high-resolution single-crystal data.

Rigaku XtaLAB Synergy, Dualflex, HyPix-Arc 100

Short Name: syn

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Structural Science

Instrument Location: KCL 11

Date of Acquisition: January 2023

\

Copper and silver X-ray sources are available. The curved image plate detector allows high dynamic range and collection out to a 2Θ angle of 144 degrees in a single image to yield high-resolution single-crystal data.

TA NanoITC

Short Name: ITC

Instrument Type: Solution Biophysics

Instrument Location: KCL 16B

Date of Acquisition: August 2014

\

By determining heat changes associated with binding between any two biomolecules, ITC measures all binding parameters in a single label-free, in-solution experiment.

TCSPC TD-Fluor Horiba Fluorolog 3 Time Domain Fluorimeter

Short Name: TCSPC

Instrument Type: Solution Biophysics

Instrument Location: KCL 16B

Date of Acquisition: December 2009

\

The Fluorolog 3 is a modular fluorimeter that can be set up to make time-domain fluorescence measurements at specific wavelengths, or steady-state fluorescence measurements over a range of excitation and/or emission wavelengths.

TTP Lab-tech Nanoliter Pipetting System

Short Name: Mosquito

Instrument Type: Structural Science

Instrument Location: SCL 26

Date of Acquisition: October 2009

\

TTP Labtech’s mosquito allows for the creation of protein crystallization screens with several multi-component drops per well, even in 96-well hanging drop set-ups.

UV-VIS Cary 3E Spectrophotometer

Short Name: UV-VIS

Instrument Type: Optical Spectroscopy

Instrument Location: KCL 3

Date of Acquisition: November 1995

\

Standard cuvette holder, temperature-regulated cuvette sample change, and integrating sphere available.

Standard Operating Procedure

UV-VIS Shimadzu UV-3600Plus

Short Name: UV-3600

Instrument Type: Optical Spectroscopy

Instrument Location: KCL 3

Date of Acquisition: February 2020

\

High performance UV-VIS-NIR Spectrophotometer with four gratings, wide dynamic range -6 to 6.0 ABS, resolution to 0.1nm, excellent stray light rejection: less than 0.00008% at 220nm, sealed optics, self-aligning lamps, high-mass, stable optical bench, selectable and constant bandpass.

Options:

V600a Varian VNMRS 600 MHz NMR Spectrometer (Manual)

Short Name: V600a

Instrument Type: NMR & EPR

Instrument Location: CRB 100

Date of Acquisition: July 2009

\

3 channel system. Inverse-detect HCN probe installed.

V600b Varian VNMRS 600 MHz NMR Spectrometer (Manual)

Short Name: V600b

Instrument Type: NMR & EPR

Instrument Location: CRB 100

Date of Acquisition: August 2002

\

3 channel system. Inverse-detect HCN probe installed.

V700 Varian Inova 700 MHz NMR Spectrometer (Manual)

Short Name: V300b

Instrument Type: NMR & EPR

Instrument Location: KCL 3

Date of Acquisition: October 2010

\

4 channel system, triple resonance probe normally installed.

Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System

Short Name: SAXSWAXS

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Scattering, Instrument Type: Solution Biophysics, Instrument Type: Structural Science

Instrument Location: SCL 26

Date of Acquisition: December 2022

\

The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.

In particular, the following key features enable the user to optimize experiments or results:

Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System

Short Name: SAXSWAXS

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Scattering, Instrument Type: Solution Biophysics, Instrument Type: Structural Science

Instrument Location: SCL 26

Date of Acquisition: December 2022

\

The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.

In particular, the following key features enable the user to optimize experiments or results:

Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System

Short Name: SAXSWAXS

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Scattering, Instrument Type: Solution Biophysics, Instrument Type: Structural Science

Instrument Location: SCL 26

Date of Acquisition: December 2022

\

The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.

In particular, the following key features enable the user to optimize experiments or results:

Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System

Short Name: SAXSWAXS

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Scattering, Instrument Type: Solution Biophysics, Instrument Type: Structural Science

Instrument Location: SCL 26

Date of Acquisition: December 2022

\

The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.

In particular, the following key features enable the user to optimize experiments or results:

XT H 225 Computed Tomography Scanner

Short Name: microCT

Instrument Type: Imaging, Instrument Type: Structural Science

Instrument Location: SCL 26

Date of Acquisition: May 2018

\

Micro-CT is a 3D imaging technique utilizing X-rays to see inside an object, slice by slice.  Micro-CT, also called microtomography or micro computed tomography, is similar to hospital CT or “CAT” scan imaging but on a small scale with greatly increased resolution. Samples can be imaged with pixel sizes as small as ~4 micron and objects can be scanned up to ~50 cm in diameter.

The versatile XT H 225 scanner can be used to cover a wide range of applications, including the inspection of plastic parts, electronics and complex mechanisms as well as researching materials and natural specimens.

The scanner can be fit with a rotating anode, reflection, or transmission target. The rotating anode produces the most X-rays, which is good for highly absorbing material, but has the lowest resolution. The transmission target has the best resolution, but produces the least amount of X-rays. The reflection target is typically installed and has a balance between X-ray intensity and resolution.  If your needs require the rotating or transmission target, please inquire in advance about scheduling.

XT H 225 Computed Tomography Scanner

Short Name: microCT

Instrument Type: Imaging, Instrument Type: Structural Science

Instrument Location: SCL 26

Date of Acquisition: May 2018

\

Micro-CT is a 3D imaging technique utilizing X-rays to see inside an object, slice by slice.  Micro-CT, also called microtomography or micro computed tomography, is similar to hospital CT or “CAT” scan imaging but on a small scale with greatly increased resolution. Samples can be imaged with pixel sizes as small as ~4 micron and objects can be scanned up to ~50 cm in diameter.

The versatile XT H 225 scanner can be used to cover a wide range of applications, including the inspection of plastic parts, electronics and complex mechanisms as well as researching materials and natural specimens.

The scanner can be fit with a rotating anode, reflection, or transmission target. The rotating anode produces the most X-rays, which is good for highly absorbing material, but has the lowest resolution. The transmission target has the best resolution, but produces the least amount of X-rays. The reflection target is typically installed and has a balance between X-ray intensity and resolution.  If your needs require the rotating or transmission target, please inquire in advance about scheduling.

XtaLAB Synergy-ED

Short Name: Synergy-ED

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Imaging, Instrument Type: Scattering, Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: January 2025

\

The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.

Key Features

Wavelength:

Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.

Temperature Range:

Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.

Viable Grain Size:

Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.

Sample Types

The Synergy-ED is optimized for a broad range of crystalline materials, including:

Applications

The Synergy-ED provides critical structural insights across multiple fields, including:

Pharmaceutical Research:

Materials Science & Nanotechnology:

Heterogeneous Sample Analysis:

XtaLAB Synergy-ED

Short Name: Synergy-ED

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Imaging, Instrument Type: Scattering, Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: January 2025

\

The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.

Key Features

Wavelength:

Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.

Temperature Range:

Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.

Viable Grain Size:

Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.

Sample Types

The Synergy-ED is optimized for a broad range of crystalline materials, including:

Applications

The Synergy-ED provides critical structural insights across multiple fields, including:

Pharmaceutical Research:

Materials Science & Nanotechnology:

Heterogeneous Sample Analysis:

XtaLAB Synergy-ED

Short Name: Synergy-ED

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Imaging, Instrument Type: Scattering, Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: January 2025

\

The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.

Key Features

Wavelength:

Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.

Temperature Range:

Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.

Viable Grain Size:

Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.

Sample Types

The Synergy-ED is optimized for a broad range of crystalline materials, including:

Applications

The Synergy-ED provides critical structural insights across multiple fields, including:

Pharmaceutical Research:

Materials Science & Nanotechnology:

Heterogeneous Sample Analysis:

XtaLAB Synergy-ED

Short Name: Synergy-ED

Instrument Type: Diffraction (X-ray and electron), Instrument Type: Imaging, Instrument Type: Scattering, Instrument Type: Structural Science

Instrument Location: KCL 7

Date of Acquisition: January 2025

\

The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.

Key Features

Wavelength:

Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.

Temperature Range:

Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.

Viable Grain Size:

Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.

Sample Types

The Synergy-ED is optimized for a broad range of crystalline materials, including:

Applications

The Synergy-ED provides critical structural insights across multiple fields, including:

Pharmaceutical Research:

Materials Science & Nanotechnology:

Heterogeneous Sample Analysis: