# Yale University
**Source**: https://cbic.yale.edu/instruments-listing
**Parent**: https://cbic.yale.edu/instruments
# Instrument Catalog
Instrument Type
- Any -Diffraction (X-ray and electron)ImagingMass SpectrometryNMR & EPROptical SpectroscopyScatteringSolution BiophysicsStructural Science
Instrument Location
- Any -CRB 100KCL 3KCL 7KCL 11KCL 12KCL 16BSCL 148SCL 136SCL 123SCL 123aSCL 26
Sort by
Instrument NameDate of AcquisitionInstrument Type
Order
AscDesc
\*\*Chemical Crystallography User Policy\*\*
**Short Name:** Small Molecule Diffraction Policy
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** September 2013
\
***Co-authorship suggestions***
Please note that single crystal diffraction/model prices do not fluctuate based on effort or time and are not waived even if the diffraction model warrants co-authorship. Should you publish data collected in our center, please acknowledge our facility in your manuscripts or grant applications as follows:
*This research made use of the Chemical and Biophysical Instrumentation Center at Yale University (RRID:SCR\_021738).*
Dr. Mercado is available to assist with the diffraction components of experiments and review manuscripts. Co-authorship should be considered if:
1. The structural information in the report is derived primarily from diffraction data and constitutes a significant part of the writing.
2. The structure determination is non-routine, such as involving variable temperature studies, phase changes, or specialized collection environments.
3. The final structure required a substantial amount of time and technical expertise. For instance:
- Problematic twinning or significant disorder in structure refinement.
- Careful consideration of anomalous dispersion (e.g., absolute stereochemistry).
- Determination of the model by MicroED.
***Center Use Policy***
- Staff mounted samples will be mounted at the convenience of their schedule.
- Samples can be submitted on the table outside KCL 3
- To independently use the single crystal diffractometers, you must meet one of the following subpoints:
- Completed an instrument orientation workshop held in the CBIC (~4 hour time commitment)
- Completed CHEM 511 and 512
- Completed an equivalent course/training at a different institution with a recommendation from the instructor/instrument manager.
- Independent access enables X-ray facility use for your own research.
- Independent use does not enable facility use for you to collect data on behalf of other researchers at Yale.
- Exceptions are granted on a case-by-case basis.
\*\*Chemical Crystallography User Policy\*\*
**Short Name:** Small Molecule Diffraction Policy
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** September 2013
\
***Co-authorship suggestions***
Please note that single crystal diffraction/model prices do not fluctuate based on effort or time and are not waived even if the diffraction model warrants co-authorship. Should you publish data collected in our center, please acknowledge our facility in your manuscripts or grant applications as follows:
*This research made use of the Chemical and Biophysical Instrumentation Center at Yale University (RRID:SCR\_021738).*
Dr. Mercado is available to assist with the diffraction components of experiments and review manuscripts. Co-authorship should be considered if:
1. The structural information in the report is derived primarily from diffraction data and constitutes a significant part of the writing.
2. The structure determination is non-routine, such as involving variable temperature studies, phase changes, or specialized collection environments.
3. The final structure required a substantial amount of time and technical expertise. For instance:
- Problematic twinning or significant disorder in structure refinement.
- Careful consideration of anomalous dispersion (e.g., absolute stereochemistry).
- Determination of the model by MicroED.
***Center Use Policy***
- Staff mounted samples will be mounted at the convenience of their schedule.
- Samples can be submitted on the table outside KCL 3
- To independently use the single crystal diffractometers, you must meet one of the following subpoints:
- Completed an instrument orientation workshop held in the CBIC (~4 hour time commitment)
- Completed CHEM 511 and 512
- Completed an equivalent course/training at a different institution with a recommendation from the instructor/instrument manager.
- Independent access enables X-ray facility use for your own research.
- Independent use does not enable facility use for you to collect data on behalf of other researchers at Yale.
- Exceptions are granted on a case-by-case basis.
500wb Bruker Neo 500 MHz NMR spectrometer
**Short Name:** V500wb
**Instrument Type:** NMR & EPR
**Instrument Location:** CRB 100
**Date of Acquisition:** December 1995
\
500 MHz wide-bore NMR spectrometer.
3.2 mm CPMAS probe normally installed for solid-state NMR experiments.
Micro MRI imaging capabilities
A400a Agilent DD2 400 MHz NMR Spectrometer (Autosampler)
**Short Name:** A400a
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 3
**Date of Acquisition:** August 2013
\
2 channel system with AS7620 96-sample changer. OneNMR\
probe with auto-tune-and-match installed.
Undergraduates have priority on this instrument during lab periods.
[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)
Best experiments:
- PROTON
- COSY
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
Acceptable experiments:
- C13CPD (night time)
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
Not available:
- Variable Temperature experiments
- Kinetics experiments
- Simultaneous F19 and H1 experiments
**Time Required for NMR Experiments on A400a:**
| | | | | | | |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 10 h 40 min | 35389 hours | 1785 hours | 1886 hours | 7129 hours |
| 0.06 mg | 0.2 mM | 2 h 40 min | 10516 hours | 46 h 28 min | 474 h 12 min | 1785 hours |
| 0.15 mg | 0.5 mM | 0 h 26 min | 1415 h 33 min | 6 h 10 min | 75 h 01 min | 284 h 44 min |
| 0.3 mg | 1 mM | 0 h 06 min | 354 h 01 min | 1 h 40 min | 18 h 53 min | 71 h 19 min |
| 0.6 mg | 2 mM | 0 h 01 min | 88 h 31 min | 0 h 26 min | 4 h 42 min | 17 h 51 min |
| 1.5 mg | 5 mM | < 1 min | 14 h 09 min | 0 h 03 min | 0 h 45 min | 2 h 51 min |
| 3 mg | 10 mM | < 1 min | 3 h 32 min | 0 h 03 min | 0 h 16 min | 1 h 07 min |
| 6 mg | 20 mM | < 1 min | 0 h 53 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 13 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 08 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
A400b Agilent DD2 400 MHz NMR Spectrometer (Manual)
**Short Name:** A400b
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 3
**Date of Acquisition:** October 2013
\
2 channel system. AutoX DB probe installed. Variable-temperature and H/F experiments available.
[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)
Best experiments:
- PROTON
- Simultaneous F19 and H1 experiments (i.e. F19 with H1 decoupling)
- Variable Temperature experiments (with appropriate CBIC training)
- Kinetics experiments
Acceptable experiments:
- C13CPD
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
Not available:
- Nuclei other than H1, F19 & C13
**Time Required for NMR Experiments on A400b:**
| | | | | | | |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 44 h 44 min | 50960 hours | 644 hours | 7862 hours | 29844 hours |
| 0.06 mg | 0.2 mM | 11 h 11 min | 12740 hours | 161 hours | 1965 hours | 7461 hours |
| 0.15 mg | 0.5 mM | 1 h 47 min | 2038 h 24 min | 25 h 47 min | 319 h 18 min | 1193 h 57 min |
| 0.3 mg | 1 mM | 0 h 27 min | 509 h 36 min | 6 h 30 min | 78 h 37 min | 298 h 40 min |
| 0.6 mg | 2 mM | 0 h 07 min | 127 h 27 min | 1 h 38 min | 19 h 48 min | 75 h 40 min |
| 1.5 mg | 5 mM | 0 h 01 min | 20 h 23 min | 0 h 16 min | 3 h 08 min | 12 h 15 min |
| 3 mg | 10 mM | < 1 min | 5 h 15 min | 0 h 06 min | 0 h 47 min | 1 h 32 min |
| 6 mg | 20 mM | < 1 min | 1 h 16 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 19 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 12 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
A400c Agilent DD2 400 MHz NMR Spectrometer (Autosampler)
**Short Name:** A400c
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 3
**Date of Acquisition:** October 2013
\
2 channel system with AS7620 96-sample changer. OneNMR probe with auto-tune-and-match installed.
Primarily used for short, routine NMR experiments where data is needed quickly.
[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)
Best experiments:
- PROTON
- COSY
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
Acceptable experiments:
- C13CPD (night time)
- 2D HSQC and HMBC experiments (night time)
- 1D/2D NOE experiments (night time)
Not available:
- Variable Temperature experiments
- Kinetics experiments
- Simultaneous F19 and H1 experiments
**Time Required for NMR Experiments on A400c:**
| | | | | | | |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 10 h 40 min | 35389 hours | 1785 hours | 1886 hours | 7129 hours |
| 0.06 mg | 0.2 mM | 2 h 40 min | 10516 hours | 46 h 28 min | 474 h 12 min | 1785 hours |
| 0.15 mg | 0.5 mM | 0 h 26 min | 1415 h 33 min | 6 h 10 min | 75 h 01 min | 284 h 44 min |
| 0.3 mg | 1 mM | 0 h 06 min | 354 h 01 min | 1 h 40 min | 18 h 53 min | 71 h 19 min |
| 0.6 mg | 2 mM | 0 h 01 min | 88 h 31 min | 0 h 26 min | 4 h 42 min | 17 h 51 min |
| 1.5 mg | 5 mM | < 1 min | 14 h 09 min | 0 h 03 min | 0 h 45 min | 2 h 51 min |
| 3 mg | 10 mM | < 1 min | 3 h 32 min | 0 h 03 min | 0 h 16 min | 1 h 07 min |
| 6 mg | 20 mM | < 1 min | 0 h 53 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 13 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 08 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
A500a Agilent DD2 500 MHz NMR Spectrometer (Manual)
**Short Name:** A500a
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 3
**Date of Acquisition:** January 2014
\
2 channel system, OneNMR probe with auto-tune-and-match installed.
[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)
Best experiments:
- PROTON
- COSY
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
- Variable Temperature experiments
- Kinetics experiments
Acceptable experiments:
- C13CPD
Not available:
- Simultaneous F19 and H1 experiments
**Time Required for NMR Experiments on A500a:**
| | | | | | | |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 5 hours | 16962 hours | 67 h 16 min | 820 h 42 min | 3113 h 37 min |
| 0.06 mg | 0.2 mM | 1 h 10 min | 424 h 02 min | 16 h 50 min | 205 h 15 min | 779 h 06 min |
| 0.15 mg | 0.5 mM | 0 h 11 min | 678 h 28 min | 2 h 45 min | 32 h 50 min | 124 h 34 min |
| 0.3 mg | 1 mM | 0 h 03 min | 170 h 00 min | 0 h 42 min | 8 h 20 min | 31 h 09 min |
| 0.6 mg | 2 mM | < 1 min | 4 h 15 min | 0 h 11 min | 2 h 05 min | 7 h 48 min |
| 1.5 mg | 5 mM | < 1 min | 3 h 11 min | 0 h 03 min | 0 h 08 min | 1 h 40 min |
| 3 mg | 10 mM | < 1 min | 1 h 42 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 25 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 06 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
A500b Agilent DD2 500 MHz NMR Spectrometer (Manual)
**Short Name:** A500b
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 3
**Date of Acquisition:** September 2012
\
2 channel system, OneNMR probe with auto-tune-and-match installed.
[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)
Best experiments:
- PROTON
- COSY
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
- Variable Temperature experiments
- Kinetics experiments
Acceptable experiments:
- C13CPD
Not available:
- Simultaneous F19 and H1 experiments
**Time Required for NMR Experiments on A500b:**
| | | | | | | |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 5 hours | 16962 hours | 67 h 16 min | 820 h 42 min | 3113 h 37 min |
| 0.06 mg | 0.2 mM | 1 h 10 min | 424 h 02 min | 16 h 50 min | 205 h 15 min | 779 h 06 min |
| 0.15 mg | 0.5 mM | 0 h 11 min | 678 h 28 min | 2 h 45 min | 32 h 50 min | 124 h 34 min |
| 0.3 mg | 1 mM | 0 h 03 min | 170 h 00 min | 0 h 42 min | 8 h 20 min | 31 h 09 min |
| 0.6 mg | 2 mM | < 1 min | 4 h 15 min | 0 h 11 min | 2 h 05 min | 7 h 48 min |
| 1.5 mg | 5 mM | < 1 min | 3 h 11 min | 0 h 03 min | 0 h 08 min | 1 h 40 min |
| 3 mg | 10 mM | < 1 min | 1 h 42 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 25 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 06 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
A600a Agilent DD2 600 MHz NMR Spectrometer (Autosampler)
**Short Name:** A600a
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 3
**Date of Acquisition:** October 2013
\
3 channel system with AS7510 12-sample automatic sample changer. C{H} cold probe with auto-tune-and-match installed. Highest sensitivity for C13 NMR experiments.
[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)
Best experiments:
- CARBON
- PROTON
Acceptable experiments:
- 1D/2D NOE experiments
Not available:
- Variable Temperature experiments
- Kinetics experiments
- Nuclei other than H1 and C13
**Time Required for NMR Experiments on A600a:**
| | | | | | | |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 0 h 24 min | 1298 hours | 5 h 54 min | 72 h 04 min | 272 h 22 min |
| 0.06 mg | 0.2 mM | 0 h 06 min | 324 h 32 min | 1 h 28 min | 19 h 35 min | 68 h 32 min |
| 0.15 mg | 0.5 mM | 0 h 01 min | 51 h 56 min | 0 h 16 min | 3 h 11 min | 11 h 10 min |
| 0.3 mg | 1 mM | < 1 min | 13 h 00 min | 0 h 03 min | 0 h 47 min | 2 h 47 min |
| 0.6 mg | 2 mM | < 1 min | 3 h 15 min | 0 h 03 min | 0 h 16 min | 1 h 07 min |
| 1.5 mg | 5 mM | < 1 min | 0 h 31 min | 0 h 03 min | 0 h 08 min | 0 h 34 min |
| 3 mg | 10 mM | < 1 min | 0 h 08 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube
A800 Agilent 800 MHz NMR Spectrometer (Manual)
**Short Name:** A800
**Instrument Type:** NMR & EPR
**Instrument Location:** CRB 100
**Date of Acquisition:** July 2013
\
4 channel system. Inverse-detect HCN probe, Dual Band (direct-detect) probe, and H{CN} C13-sensitivity enhanced salt-tolerant cold probe available.
Experienced Users Only. Contact CBIC staff for more information.
Analytical Ultracentrifuge Beckman Coulter Proteomelab XL-I
**Short Name:** AU
**Instrument Type:** Solution Biophysics
**Instrument Location:** KCL 3
**Date of Acquisition:** November 2009
\
Analytical ultracentrifugation is a column-free separation technique that measures the relative change in the distribution of molecular weights, providing a way to measure heterogeneity, stoichiometry and self-association of proteins, oligomers, aggregates, particles, colloids, and small structures in solution.
Applied Photophysics Chirascan Circular Dichroism Spectrometer
**Short Name:** CD
**Instrument Type:** Solution Biophysics
**Instrument Location:** KCL 3
**Date of Acquisition:** September 2013
\
Chirascan is a modern, high performance circular dichroism (CD) spectrometer operating in the ultraviolet and visible wavelength range. It is typically used to probe the secondary structure or conformation of bio-molecules.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/CD%20SOP.pdf)
DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II
**Short Name:** DLS
**Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science
**Instrument Location:** KCL 3
**Date of Acquisition:** October 2013
\
Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.
Typical applications for DLS include:
- Screening macromolecules prior to crystallization trials
- Measuring the stability of protein samples for NMR analysis
- Monitoring molecular aggregation
- Characterizing nanoparticles
The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/DLS%20SOP.pdf)
DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II
**Short Name:** DLS
**Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science
**Instrument Location:** KCL 3
**Date of Acquisition:** October 2013
\
Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.
Typical applications for DLS include:
- Screening macromolecules prior to crystallization trials
- Measuring the stability of protein samples for NMR analysis
- Monitoring molecular aggregation
- Characterizing nanoparticles
The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/DLS%20SOP.pdf)
DLS Dynamic Light Scattering Wyatt DynaPro PlateReader II
**Short Name:** DLS
**Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science
**Instrument Location:** KCL 3
**Date of Acquisition:** October 2013
\
Dynamic light scattering measures the diffusion rate of molecules or particles in solution by observing the fluctuations in scattered light from a laser passing through a sample. The sensitivity of DLS increases with increasing molecular size, with a typical lower size limit of 0.5 nanometers and upper size limit of 10 micrometers.
Typical applications for DLS include:
- Screening macromolecules prior to crystallization trials
- Measuring the stability of protein samples for NMR analysis
- Monitoring molecular aggregation
- Characterizing nanoparticles
The DynaPro PlateReader II is set up to make these measurements in 96, 384, or 1536 well plates using as little as 5 microliters of sample.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/DLS%20SOP.pdf)
EPR Bruker EleXsys EPR Spectrometer
**Short Name:** EPR
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 12
**Date of Acquisition:** September 2000
\
X-Band Electron Paramagnetic Resonance. Equipped with a cryogen-free cryostat.
FTIR ASI React IR Reaction Monitoring System
**Short Name:** ReactIR
**Instrument Type:** Optical Spectroscopy
**Instrument Location:** KCL 3
**Date of Acquisition:** June 1998
\
React IR 15 FTIR for real-time monitoring of chemical reactions in situ.
FTIR Shimadzu IRTracer-100
**Short Name:** IR Tracer
**Instrument Type:** Optical Spectroscopy
**Instrument Location:** KCL 3
**Date of Acquisition:** February 2020
\
The IRTracer-100 offers exceptionally high sensitivity with an SN ratio of 60,000:1, high resolution down to 0.25 cm−1, and fast measurement speeds up to 20 scans per second.\
The system supports a wide variety of applications with features such as a 12,000-entry library and a data analysis program for contaminant analysis and time course and rapid scan functions for tracking reactions.
Available Accessories:
- ATR Unit for Solid Samples
- VeeMax II Variable Angle Specular Reflectance Accessory
- Gas Cuvette & Holder
- KBr Pellets
For more compatible accessories for your measurement needs, please have a look at the [Shimadzu Selection Guide](https://www.ssi.shimadzu.com/products/ftir-spectrophotometers/ftir-accessory-selection-guide.html).
FTIR/Raman Thermo Nicolet 6700
**Short Name:** FTIR
**Instrument Type:** Optical Spectroscopy
**Instrument Location:** KCL 3
**Date of Acquisition:** November 2006
\
Diamond ATR cell normally installed. Transmission, DRIFTS, ZnSe ATR, and high-temperature accessories available. Raman with IR-laser source also available.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/FTIR%20SOP.pdf)
GC-MS Agilent 6890N/5973
**Short Name:** GC-MS
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** February 2004
\
Open access GC-MS instrument with 100-position sample changer and 20 meter column installed.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/GCMS%20SOP.pdf)
GC-MS column selection
**Short Name:** GC-MS columns
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** September 2021
\
| | | | | | |
| --- | --- | --- | --- | --- | --- |
| **Column Name** | **Manufacturer** | **Thickness (um)** | **Length (m)** | **Diameter (mm)** | **Max. Temp. (deg C)** |
| SH-Rxi-5Sil MS | Shimadzu | 0.25 | 30 | 0.25 | 350 |
| DB select 624UI | Agilent | 1.4 | 30 | 0.25 | 260 |
| ZB-WAX | Zebron | 0.5 | 30 | 0.25 | 250 |
| PoraPLOT Q | Agilent | 8 | 25 | 0.25 | 250 |
| EC-1 | Grace | 0.25 | 30 | 0.25 | 350 |
| VF-WAXms | Agilent | 1 | 30 | 0.25 | 260 |
| Rtx-1 | Restek | 0.25 | 15 | 0.25 | 350 |
| Stabilwax-MS | Restek | 0.25 | 30 | 0.25 | 260 |
GC-TQ-MS Shimadzu 8050 NX
**Short Name:** GC-TQ-MS
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** December 2019
\
[**Shimadzu 8050 NX Triple Quadrupole (TQ) GC-MS**](https://www.ssi.shimadzu.com/products/gas-chromatography-mass-spectrometry/gcms-tq8050-nx.html) with Liquid Injection, Solid Phase Micro Extraction (SPME) and Headspace Analysis Autosampler. Triple Quadrupole MS instruments allow for quantitation of compounds, collision induced dissociation (CID) and multiple reaction monitoring (MRM). The system is equipped with an Electron Ionization (EI) source, a Chemical Ionization (CI) source and a Flame Ionization Detector (FID).
LC-MS Shimadzu 8060 TQ
**Short Name:** 8060
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** January 2020
\
Service only [**Shimadzu LCMS-8060**](https://www.ssi.shimadzu.com/products/liquid-chromatography-mass-spectrometry/lcms-8060.html) Triple Quadrupole Liquid Chromatograph Mass Spectrometer (LC-MS/MS). Triple Quadrupole MS instruments allow for quantitation of compounds, collision induced dissociation (CID) and multiple reaction monitoring (MRM) with high sensitivity. The UPLC system includs a fluidic system with two different solvents, three trays for up to 54 samples each (in GC vials), three UPLC columns (see list below) and a photo-diode array (UV/Vis) detector. 96 and 384 well plates can be used for screening projects, please reach out first.
Column 1: Shim-pack Scepter C18-120 1.9mum - 2.1 x 50mm\
Column 2: Shim-pack Scepter C18-120 1.9mum - 2.1 x 100mm\
Column 3: Shim-pack Scepter C8-120 1.9mum - 2.1 x 100mm
Different columns can be installed on demand.
Standard Solvents available for LC separation:
Water (with 0.1% Formic Acid or 10mM NH4Ac)\
Methanol (with and w/o 0.1% Formic Acid or 10mM NH4Ac)\
Acetonitrile (with and w/o 0.1% Formic Acid or 10mM NH4Ac)
Different solvents can be used on demand.
LC-MS Shimadzu 9030 Quadrupole Time-of-Flight MS
**Short Name:** 9030
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** January 2020
\
[**Shimadzu LCMS-9030**](https://www.ssi.shimadzu.com/products/liquid-chromatography-mass-spectrometry/lcms-9030.html) Quadrupole Time-of-Flight High-Performance Liquid Chromatograph Mass Spectrometer for high-resolution MS.
Service only high resolution Q-tof MS instrument with an UPLC system, including a fluidic system with two different solvents, three trays for up to 54 samples each (in GC vials), three UPLC columns (see list below) and a photo-diode array (UV/Vis) detector. 96 and 384 well plates can be used for screening projects, please reach out first.
Column 1: Shim-pack Scepter C18-120 1.9mum - 2.1 x 50mm\
Column 2: Shim-pack Scepter C18-120 1.9mum - 2.1 x 100mm\
Column 3: Shim-pack Scepter C8-120 1.9mum - 2.1 x 100mm
Different columns can be installed on demand.
Standard Solvents available for LC separation:
- Water (with 0.1% Formic Acid or 10mM NH4Ac)
- Methanol (with and w/o 0.1% Formic Acid or 10mM NH4Ac)
- Acetonitrile (with and w/o 0.1% Formic Acid or 10mM NH4Ac)
Different solvents can be used on demand.
LC-MS ThermoScientific QExactive Orbitrap (+ IR spectroscopy)
**Short Name:** Orbi
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** August 2019
\
Service only high resolution **[QExactive Orbitrap FT-MS](https://planetorbitrap.com/q-exactive)** instrument with an Dionex 3000 UPLC system, including a fluidic system with up to four different solvents, three trays for up to 40 samples each (in 2 mL GC vials), one UPLC column, a UV/Vis photo-diode array (PDA) and a fluorescence (FL) detector.
The structural mass spectrometry extension on this instrument allows for recording of infrared spectra of mass selected ions in the gas phase. Read more about the technique .
Software for opening and converting .raw files: <http://msfragger.nesvilab.org/tutorial_convert.html>
LC-MS Waters Xevo Qtof high-resolution MS
**Short Name:** Xevo
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** November 2018
\
Service only high resolution Q-tof MS instrument with an UPLC system, including a fluidic system with up to four different solvents, two trays for up to 48 samples each (in GC vials), four UPLC columns (see list below) and a photo-diode array (UV/Vis).
Column 1: Acquity UPLC BEH C18 1.7mum - 2.1 x 50mm\
Column 2: Acquity UPLC BEH Glycan 1.7mum - 2.1 x 100mm\
Column 3: Acquity UPLC BEH300 C18 1.7mum - 2.1 x 100mm\
Column 4: Acquity UPLC BEH C8 1.7mum - 2.1 x 100mm
MALDI-ToF-MS Shimadzu AXIMA Confidence
**Short Name:** MALDI
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** January 2020
\
[AXIMA Confidence](https://www.ssi.shimadzu.com/products/maldi-tof-mass-spectrometry/axima-confidence.html) Linear/Reflectron MALDI-TOF Mass Spectrometer from Shimadzu.
**Target Plates available:**\
AXIMA 384 well (2.8mm I.D.) plate - P/N: DE1580TA
| | |
| --- | --- |
| *Number of wells:* | *Sample = 384 Calibrant = 0* |
| *Internal diameter of wells:* | *Sample = 2.8mm Calibrant = N/A* |
FlexiMass-SR48 steel targets - P/N: TO-431R00
| | |
| --- | --- |
| *Number of wells:* | *Sample = 48 Calibrant = 3* |
| *Internal diameter of wells:* | *Sample = 2.8mm Calibrant = 2.2mm* |
FlexiMass-DS disposable targets - P/N: TO-430R00
| | |
| --- | --- |
| *Number of wells:* | *Sample = 48 Calibrant = 3* |
| *Internal diameter of wells:* | *Sample = 2.8mm Calibrant = 1.5mm* |
Mass Spectrometry meets Infrared Spectroscopy
**Short Name:** Mass Spec based CIVP IR Spectroscopy
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** December 2021
\
Mass Spectrometry meets Infrared Spectroscopy
### **Structural insights into cold, mass selected ions**
[https://cbic.yale.edu/mass-spectrometry-meets-infrared-spectroscopy](/mass-spectrometry-meets-infrared-spectroscopy)
MS recommendations, method descriptions and software
**Short Name:** MS resources
**Instrument Type:** Mass Spectrometry
**Instrument Location:** KCL 3
**Date of Acquisition:** February 2021
\
*Disclaimer: All information on this site is subject to change and the actual experimental methods used might differ from the ones found here!*
**Recommendations:**
[MS\_Sample\_Method\_Recommendations\_v2.docx](/sites/default/files/files/MS_Sample_Method_Recommendations_v2.docx)
**Method Descriptions** (methods might have changed over time, please double-check!):
[Shimadzu 9030 HRMS Description](/sites/default/files/files/Experimental-Method_Shimadzu-QToF-9030_v4.docx)
[Thermo Fisher QExactive Orbitrap HRMS Description](/sites/default/files/files/Experimental-Method_Thermo-QExactive.docx)
**Software:**
**Data exchange**
MS data can be exported as mzXML or mzML files and shared via ***box*** or ***google drive*** upon request. The files contain TIC and MS data, EIC can be extracted through user analysis. These data files can be opened with **MestreNova** (updated and fully installed licenses necessary!):
*Download the software and license bundle from the CBIC homepage:*
[*https://cbic.yale.edu/yale-only/mnova-download*](https://cbic.yale.edu/yale-only/mnova-download)
*Close MestreNova (for update of existing installation), and run as Administrator by:*
*Windows Start menu -> MestreNova Folder -> Right Click on MestreNova -> more -> Run as Administrator*
*Install the Licenses again, close the software and start it as a normal user.*
**Complex data workups** and **simple txt data** can be provided based on the project.
Software for opening and converting ThermoFisher .raw files: <http://msfragger.nesvilab.org/tutorial_convert.html>
**Acknowledgements:**
If you use CBIC instruments in your research, we request that you acknowledge our facility in your written publications or grant applications as follows:
Acknowledgement: *This research made use of the Chemical and Biophysical Instrumentation Center at Yale University (RRID:SCR\_021738). Equipment was purchased with funds from Yale University.\
Optional for Orbitrap QExactive MS: NSF MRI grant CHE-1828190.*\
Pilatus3R HPC on a 007 VHF+Arc)Sec X-ray Source
**Short Name:** 007c
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** October 2016
\
The Pilatus3R 200KA detector on our Rigaku 007 HF+ rotating anode with VHF Arc)Sec optics is suitable for solving small molecules samples. This instrument is open to usage by students and staff. λ = 0.7107 Å
Pilatus3R HPC on a 007 VHF+Arc)Sec X-ray Source
**Short Name:** 007c
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** October 2016
\
The Pilatus3R 200KA detector on our Rigaku 007 HF+ rotating anode with VHF Arc)Sec optics is suitable for solving small molecules samples. This instrument is open to usage by students and staff. λ = 0.7107 Å
Polarimeter Perkin Elmer 341
**Short Name:** Polarimeter
**Instrument Type:** Optical Spectroscopy
**Instrument Location:** KCL 16B
**Date of Acquisition:** January 1997
\
General-purpose precision Polarimeter with Na and Hg source lamps and Glan-Taylor polarizer.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/Polarimeter%20SOP.pdf)
Polarimeter Rudolph Autopol IV
**Short Name:** Autopol
**Instrument Type:** Optical Spectroscopy
**Instrument Location:** KCL 16B
**Date of Acquisition:** February 2018
\
The **AUTOPOL® IV** is a microprocessor based automatic polarimeter that is available in one, two and six wavelength versions. The AUTOPOL® IV’s features include: readout in [Specific Rotation](http://rudolphresearch.com/polarimeters-and-polarimetry/ "Specific Rotation"), [Optical Rotation](http://rudolphresearch.com/polarimeters-and-polarimetry/ "Optical Rotation"), and concentration, customer defined and labeled programs for push button readout, large LCD display and touchscreen user interface, temperature display and correction, push button wavelength selection, statistics, 3 USB ports, 1-USB 3.0, RS 232 and parallel printer ports.
Rigaku CCD on 007HF+ X-ray Source
**Short Name:** 007a
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** October 2009
\
The hybrid pixel array detector on our Rigaku 007 HF+ rotating anode X-ray source with HF optics is suitable for solving small-molecule and protein structures by single-crystal X-ray diffraction. This instrument can also be used to screen crystals before sending them to an x-ray beam line at a synchrotron. λ = 1.5418 Å
Rigaku CCD on 007HF+ X-ray Source
**Short Name:** 007a
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** October 2009
\
The hybrid pixel array detector on our Rigaku 007 HF+ rotating anode X-ray source with HF optics is suitable for solving small-molecule and protein structures by single-crystal X-ray diffraction. This instrument can also be used to screen crystals before sending them to an x-ray beam line at a synchrotron. λ = 1.5418 Å
Rigaku CCD on 007HF+Arc)Sec X-ray Source
**Short Name:** 007b
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** June 2016
\
The Rigaku Saturn 944HG CCD detector on our Rigaku 007 HF+ rotating anode X-ray source with HF Arc)Sec optics is suitable for solving small molecules samples. This instrument is run by the CBIC Staff. λ = 1.5418 Å
Rigaku CCD on 007HF+Arc)Sec X-ray Source
**Short Name:** 007b
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** June 2016
\
The Rigaku Saturn 944HG CCD detector on our Rigaku 007 HF+ rotating anode X-ray source with HF Arc)Sec optics is suitable for solving small molecules samples. This instrument is run by the CBIC Staff. λ = 1.5418 Å
Rigaku MiniFlex600 Powder X-ray Diffractometer
**Short Name:** PXRD
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 11
**Date of Acquisition:** December 2004
\
This powder diffractometer can characterize crystallinity, crystal phases, and, in many cases, the identity of solid samples. Our instrument has a sealed-tube copper X-ray source, a scintillation counter with high dynamic range, and Bragg-Brentano geometry with slits providing high resolution for flat powder samples. λ = 1.5418 Å
Rigaku MiniFlex600 Powder X-ray Diffractometer
**Short Name:** PXRD
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 11
**Date of Acquisition:** December 2004
\
This powder diffractometer can characterize crystallinity, crystal phases, and, in many cases, the identity of solid samples. Our instrument has a sealed-tube copper X-ray source, a scintillation counter with high dynamic range, and Bragg-Brentano geometry with slits providing high resolution for flat powder samples. λ = 1.5418 Å
Rigaku XtaLAB Mini II with a hybrid pixel detector
**Short Name:** mini2
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 11
**Date of Acquisition:** May 2022
\
The XtaLabMini II is a fully functional benchtop single-crystal X-ray diffractometer with a CCD detector. λ = 0.7107 Å
Rigaku XtaLAB Mini II with a hybrid pixel detector
**Short Name:** mini2
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 11
**Date of Acquisition:** May 2022
\
The XtaLabMini II is a fully functional benchtop single-crystal X-ray diffractometer with a CCD detector. λ = 0.7107 Å
Rigaku XtaLAB Synergy, Dualflex, HyPix-Arc 100
**Short Name:** syn
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 11
**Date of Acquisition:** January 2023
\
Copper and silver X-ray sources are available. The curved image plate detector allows high dynamic range and collection out to a 2Θ angle of 144 degrees in a single image to yield high-resolution single-crystal data.
Rigaku XtaLAB Synergy, Dualflex, HyPix-Arc 100
**Short Name:** syn
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Structural Science
**Instrument Location:** KCL 11
**Date of Acquisition:** January 2023
\
Copper and silver X-ray sources are available. The curved image plate detector allows high dynamic range and collection out to a 2Θ angle of 144 degrees in a single image to yield high-resolution single-crystal data.
TA NanoITC
**Short Name:** ITC
**Instrument Type:** Solution Biophysics
**Instrument Location:** KCL 16B
**Date of Acquisition:** August 2014
\
By determining heat changes associated with binding between any two biomolecules, ITC measures all binding parameters in a single label-free, in-solution experiment.
TCSPC TD-Fluor Horiba Fluorolog 3 Time Domain Fluorimeter
**Short Name:** TCSPC
**Instrument Type:** Solution Biophysics
**Instrument Location:** KCL 16B
**Date of Acquisition:** December 2009
\
The Fluorolog 3 is a modular fluorimeter that can be set up to make time-domain fluorescence measurements at specific wavelengths, or steady-state fluorescence measurements over a range of excitation and/or emission wavelengths.
TTP Lab-tech Nanoliter Pipetting System
**Short Name:** Mosquito
**Instrument Type:** Structural Science
**Instrument Location:** SCL 26
**Date of Acquisition:** October 2009
\
TTP Labtech’s mosquito allows for the creation of protein crystallization screens with several multi-component drops per well, even in 96-well hanging drop set-ups.
UV-VIS Cary 3E Spectrophotometer
**Short Name:** UV-VIS
**Instrument Type:** Optical Spectroscopy
**Instrument Location:** KCL 3
**Date of Acquisition:** November 1995
\
Standard cuvette holder, temperature-regulated cuvette sample change, and integrating sphere available.
[Standard Operating Procedure](https://cbic.yale.edu/sites/default/files/files/UV-Vis%20SOP.pdf)
UV-VIS Shimadzu UV-3600Plus
**Short Name:** UV-3600
**Instrument Type:** Optical Spectroscopy
**Instrument Location:** KCL 3
**Date of Acquisition:** February 2020
\
High performance UV-VIS-NIR Spectrophotometer with four gratings, wide dynamic range -6 to 6.0 ABS, resolution to 0.1nm, excellent stray light rejection: less than 0.00008% at 220nm, sealed optics, self-aligning lamps, high-mass, stable optical bench, selectable and constant bandpass.
Options:
- Integrating sphere for solid and liquid samples with 3 detector system includes - PMT, InGaAs and Peltier controlled PbS detectors for a superior S/N ratio.
- S-1700 Thermoelectric Single Cell Holder Temperature Controller (0 to 110 deg C) with stirrer, Thermal Melt Software for analysis of e.g. DNA melting temperatures.
V600a Varian VNMRS 600 MHz NMR Spectrometer (Manual)
**Short Name:** V600a
**Instrument Type:** NMR & EPR
**Instrument Location:** CRB 100
**Date of Acquisition:** July 2009
\
3 channel system. Inverse-detect HCN probe installed.
V600b Varian VNMRS 600 MHz NMR Spectrometer (Manual)
**Short Name:** V600b
**Instrument Type:** NMR & EPR
**Instrument Location:** CRB 100
**Date of Acquisition:** August 2002
\
3 channel system. Inverse-detect HCN probe installed.
V700 Varian Inova 700 MHz NMR Spectrometer (Manual)
**Short Name:** V300b
**Instrument Type:** NMR & EPR
**Instrument Location:** KCL 3
**Date of Acquisition:** October 2010
\
4 channel system, triple resonance probe normally installed.
Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System
**Short Name:** SAXSWAXS
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science
**Instrument Location:** SCL 26
**Date of Acquisition:** December 2022
\
#### The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.
In particular, the following key features enable the user to optimize experiments or results:
- High flux settings adapted for fast kinetics embedded in a low background camera
- Largest surface of detection moveable all the way from WAXS to long distance SAXS to optimize resolution and signal-to-noise
- Long distance SAXS settings for measuring large characteristic dimensions (up to 900 nm depending on the Xeuss 3.0 model)
- Optional USAXS module to characterize large structures ( > 2.5 µm)
Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System
**Short Name:** SAXSWAXS
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science
**Instrument Location:** SCL 26
**Date of Acquisition:** December 2022
\
#### The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.
In particular, the following key features enable the user to optimize experiments or results:
- High flux settings adapted for fast kinetics embedded in a low background camera
- Largest surface of detection moveable all the way from WAXS to long distance SAXS to optimize resolution and signal-to-noise
- Long distance SAXS settings for measuring large characteristic dimensions (up to 900 nm depending on the Xeuss 3.0 model)
- Optional USAXS module to characterize large structures ( > 2.5 µm)
Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System
**Short Name:** SAXSWAXS
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science
**Instrument Location:** SCL 26
**Date of Acquisition:** December 2022
\
#### The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.
In particular, the following key features enable the user to optimize experiments or results:
- High flux settings adapted for fast kinetics embedded in a low background camera
- Largest surface of detection moveable all the way from WAXS to long distance SAXS to optimize resolution and signal-to-noise
- Long distance SAXS settings for measuring large characteristic dimensions (up to 900 nm depending on the Xeuss 3.0 model)
- Optional USAXS module to characterize large structures ( > 2.5 µm)
Xenocs Xeuss 3.0 Small/Wide X-ray Scattering System
**Short Name:** SAXSWAXS
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Scattering, **Instrument Type:** Solution Biophysics, **Instrument Type:** Structural Science
**Instrument Location:** SCL 26
**Date of Acquisition:** December 2022
\
#### The Xeuss 3.0 offers a maximum flexibility of measurement configurations to get the best possible data quality on any type of sample.
In particular, the following key features enable the user to optimize experiments or results:
- High flux settings adapted for fast kinetics embedded in a low background camera
- Largest surface of detection moveable all the way from WAXS to long distance SAXS to optimize resolution and signal-to-noise
- Long distance SAXS settings for measuring large characteristic dimensions (up to 900 nm depending on the Xeuss 3.0 model)
- Optional USAXS module to characterize large structures ( > 2.5 µm)
XT H 225 Computed Tomography Scanner
**Short Name:** microCT
**Instrument Type:** Imaging, **Instrument Type:** Structural Science
**Instrument Location:** SCL 26
**Date of Acquisition:** May 2018
\
Micro-CT is a 3D imaging technique utilizing X-rays to see inside an object, slice by slice. Micro-CT, also called microtomography or micro computed tomography, is similar to hospital CT or “CAT” scan imaging but on a small scale with greatly increased resolution. Samples can be imaged with pixel sizes as small as ~4 micron and objects can be scanned up to ~50 cm in diameter.
The versatile XT H 225 scanner can be used to cover a wide range of applications, including the inspection of plastic parts, electronics and complex mechanisms as well as researching materials and natural specimens.
The scanner can be fit with a rotating anode, reflection, or transmission target. The rotating anode produces the most X-rays, which is good for highly absorbing material, but has the lowest resolution. The transmission target has the best resolution, but produces the least amount of X-rays. The reflection target is typically installed and has a balance between X-ray intensity and resolution. If your needs require the rotating or transmission target, please inquire in advance about scheduling.
XT H 225 Computed Tomography Scanner
**Short Name:** microCT
**Instrument Type:** Imaging, **Instrument Type:** Structural Science
**Instrument Location:** SCL 26
**Date of Acquisition:** May 2018
\
Micro-CT is a 3D imaging technique utilizing X-rays to see inside an object, slice by slice. Micro-CT, also called microtomography or micro computed tomography, is similar to hospital CT or “CAT” scan imaging but on a small scale with greatly increased resolution. Samples can be imaged with pixel sizes as small as ~4 micron and objects can be scanned up to ~50 cm in diameter.
The versatile XT H 225 scanner can be used to cover a wide range of applications, including the inspection of plastic parts, electronics and complex mechanisms as well as researching materials and natural specimens.
The scanner can be fit with a rotating anode, reflection, or transmission target. The rotating anode produces the most X-rays, which is good for highly absorbing material, but has the lowest resolution. The transmission target has the best resolution, but produces the least amount of X-rays. The reflection target is typically installed and has a balance between X-ray intensity and resolution. If your needs require the rotating or transmission target, please inquire in advance about scheduling.
XtaLAB Synergy-ED
**Short Name:** Synergy-ED
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Imaging, **Instrument Type:** Scattering, **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** January 2025
\
The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.
**Key Features**
*Wavelength:*
Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.
*Temperature Range:*
Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.
*Viable Grain Size:*
Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.
**Sample Types**
The Synergy-ED is optimized for a broad range of crystalline materials, including:
- Small organic molecules (e.g., pharmaceuticals, natural products)
- Inorganic nanomaterials
- Metal-organic frameworks (MOFs) and zeolites
- Peptides and small proteins
- Polymers and hybrid materials
**Applications**
The Synergy-ED provides critical structural insights across multiple fields, including:
*Pharmaceutical Research:*
- Polymorph Studies: Determines different crystal forms of a compound, crucial for drug formulation and stability.
- Phase Transitions: Investigates temperature- or pressure-induced structural changes.
*Materials Science & Nanotechnology:*
- Grain Boundary Analysis: Characterizes defects, interfaces, and grain orientations in polycrystalline materials.
- Nanocrystalline and Amorphous Phase Identification: Helps differentiate between ordered and disordered structures.
Heterogeneous Sample Analysis:
- Sample Mixtures: Can resolve and identify multiple crystalline phases within complex mixtures.
- Solid-State Chemistry: Provides insight into phase purity and composition.
XtaLAB Synergy-ED
**Short Name:** Synergy-ED
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Imaging, **Instrument Type:** Scattering, **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** January 2025
\
The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.
**Key Features**
*Wavelength:*
Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.
*Temperature Range:*
Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.
*Viable Grain Size:*
Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.
**Sample Types**
The Synergy-ED is optimized for a broad range of crystalline materials, including:
- Small organic molecules (e.g., pharmaceuticals, natural products)
- Inorganic nanomaterials
- Metal-organic frameworks (MOFs) and zeolites
- Peptides and small proteins
- Polymers and hybrid materials
**Applications**
The Synergy-ED provides critical structural insights across multiple fields, including:
*Pharmaceutical Research:*
- Polymorph Studies: Determines different crystal forms of a compound, crucial for drug formulation and stability.
- Phase Transitions: Investigates temperature- or pressure-induced structural changes.
*Materials Science & Nanotechnology:*
- Grain Boundary Analysis: Characterizes defects, interfaces, and grain orientations in polycrystalline materials.
- Nanocrystalline and Amorphous Phase Identification: Helps differentiate between ordered and disordered structures.
Heterogeneous Sample Analysis:
- Sample Mixtures: Can resolve and identify multiple crystalline phases within complex mixtures.
- Solid-State Chemistry: Provides insight into phase purity and composition.
XtaLAB Synergy-ED
**Short Name:** Synergy-ED
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Imaging, **Instrument Type:** Scattering, **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** January 2025
\
The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.
**Key Features**
*Wavelength:*
Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.
*Temperature Range:*
Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.
*Viable Grain Size:*
Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.
**Sample Types**
The Synergy-ED is optimized for a broad range of crystalline materials, including:
- Small organic molecules (e.g., pharmaceuticals, natural products)
- Inorganic nanomaterials
- Metal-organic frameworks (MOFs) and zeolites
- Peptides and small proteins
- Polymers and hybrid materials
**Applications**
The Synergy-ED provides critical structural insights across multiple fields, including:
*Pharmaceutical Research:*
- Polymorph Studies: Determines different crystal forms of a compound, crucial for drug formulation and stability.
- Phase Transitions: Investigates temperature- or pressure-induced structural changes.
*Materials Science & Nanotechnology:*
- Grain Boundary Analysis: Characterizes defects, interfaces, and grain orientations in polycrystalline materials.
- Nanocrystalline and Amorphous Phase Identification: Helps differentiate between ordered and disordered structures.
Heterogeneous Sample Analysis:
- Sample Mixtures: Can resolve and identify multiple crystalline phases within complex mixtures.
- Solid-State Chemistry: Provides insight into phase purity and composition.
XtaLAB Synergy-ED
**Short Name:** Synergy-ED
**Instrument Type:** Diffraction (X-ray and electron), **Instrument Type:** Imaging, **Instrument Type:** Scattering, **Instrument Type:** Structural Science
**Instrument Location:** KCL 7
**Date of Acquisition:** January 2025
\
The Synergy-ED is a state-of-the-art electron diffractometer designed for Microcrystal Electron Diffraction (MicroED) / 3D Electron Diffraction (3DED) experiments. It enables high-resolution structural analysis of nanocrystalline materials that are too small for conventional X-ray diffraction techniques.
**Key Features**
*Wavelength:*
Operates at an electron wavelength of ~0.0251 Å with a 200 kV accelerating voltage, providing high-resolution diffraction data.
*Temperature Range:*
Capable of measuring samples from room temperature down to 100 K, allowing for studies of temperature-dependent structural changes.
*Viable Grain Size:*
Suitable for nanocrystals and microcrystals ranging from several hundred nanometers to less than 10 microns.
**Sample Types**
The Synergy-ED is optimized for a broad range of crystalline materials, including:
- Small organic molecules (e.g., pharmaceuticals, natural products)
- Inorganic nanomaterials
- Metal-organic frameworks (MOFs) and zeolites
- Peptides and small proteins
- Polymers and hybrid materials
**Applications**
The Synergy-ED provides critical structural insights across multiple fields, including:
*Pharmaceutical Research:*
- Polymorph Studies: Determines different crystal forms of a compound, crucial for drug formulation and stability.
- Phase Transitions: Investigates temperature- or pressure-induced structural changes.
*Materials Science & Nanotechnology:*
- Grain Boundary Analysis: Characterizes defects, interfaces, and grain orientations in polycrystalline materials.
- Nanocrystalline and Amorphous Phase Identification: Helps differentiate between ordered and disordered structures.
Heterogeneous Sample Analysis:
- Sample Mixtures: Can resolve and identify multiple crystalline phases within complex mixtures.
- Solid-State Chemistry: Provides insight into phase purity and composition.