# Yale University

**Source**: https://cbic.yale.edu/instruments-listing?field_instrument_type_value=NMR+%26+EPR
**Parent**: https://cbic.yale.edu/instruments

# Instrument Catalog

Instrument Type 

- Any -Diffraction (X-ray and electron)ImagingMass SpectrometryNMR & EPROptical SpectroscopyScatteringSolution BiophysicsStructural Science

Instrument Location 

- Any -CRB 100KCL 3KCL 7KCL 11KCL 12KCL 16BSCL 148SCL 136SCL 123SCL 123aSCL 26

Sort by 
Instrument NameDate of AcquisitionInstrument Type

Order 
AscDesc

500wb Bruker Neo 500 MHz NMR spectrometer

**Short Name:** V500wb

**Instrument Type:** NMR & EPR

**Instrument Location:** CRB 100

**Date of Acquisition:** December 1995

\

500 MHz wide-bore NMR spectrometer.

3.2 mm CPMAS probe normally installed for solid-state NMR experiments.

Micro MRI imaging capabilities

A400a Agilent DD2 400 MHz NMR Spectrometer (Autosampler)

**Short Name:** A400a

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 3

**Date of Acquisition:** August 2013

\

2 channel system with AS7620 96-sample changer. OneNMR\
probe with auto-tune-and-match installed.

Undergraduates have priority on this instrument during lab periods.

[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)

Best experiments:

- PROTON
- COSY
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)

Acceptable experiments:

- C13CPD (night time)
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments

Not available:

- Variable Temperature experiments
- Kinetics experiments
- Simultaneous F19 and H1 experiments

**Time Required for NMR Experiments on A400a:**

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 10 h 40 min | 35389 hours | 1785 hours | 1886 hours | 7129 hours |
| 0.06 mg | 0.2 mM | 2 h 40 min | 10516 hours | 46 h 28 min | 474 h 12 min | 1785 hours |
| 0.15 mg | 0.5 mM | 0 h 26 min | 1415 h 33 min | 6 h 10 min | 75 h 01 min | 284 h 44 min |
| 0.3 mg | 1 mM | 0 h 06 min | 354 h 01 min | 1 h 40 min | 18 h 53 min | 71 h 19 min |
| 0.6 mg | 2 mM | 0 h 01 min | 88 h 31 min | 0 h 26 min | 4 h 42 min | 17 h 51 min |
| 1.5 mg | 5 mM | < 1 min | 14 h 09 min | 0 h 03 min | 0 h 45 min | 2 h 51 min |
| 3 mg | 10 mM | < 1 min | 3 h 32 min | 0 h 03 min | 0 h 16 min | 1 h 07 min |
| 6 mg | 20 mM | < 1 min | 0 h 53 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 13 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 08 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A400b Agilent DD2 400 MHz NMR Spectrometer (Manual)

**Short Name:** A400b

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 3

**Date of Acquisition:** October 2013

\

2 channel system. AutoX DB probe installed.  Variable-temperature and H/F experiments available.

[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)

Best experiments:

- PROTON
- Simultaneous F19 and H1 experiments (i.e. F19 with H1 decoupling)
- Variable Temperature experiments (with appropriate CBIC training)
- Kinetics experiments

Acceptable experiments:

- C13CPD
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments

Not available:

- Nuclei other than H1, F19 & C13

**Time Required for NMR Experiments on A400b:**

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 44 h 44 min | 50960 hours | 644 hours | 7862 hours | 29844 hours |
| 0.06 mg | 0.2 mM | 11 h 11 min | 12740 hours | 161 hours | 1965 hours | 7461 hours |
| 0.15 mg | 0.5 mM | 1 h 47 min | 2038 h 24 min | 25 h 47 min | 319 h 18 min | 1193 h 57 min |
| 0.3 mg | 1 mM | 0 h 27 min | 509 h 36 min | 6 h 30 min | 78 h 37 min | 298 h 40 min |
| 0.6 mg | 2 mM | 0 h 07 min | 127 h 27 min | 1 h 38 min | 19 h 48 min | 75 h 40 min |
| 1.5 mg | 5 mM | 0 h 01 min | 20 h 23 min | 0 h 16 min | 3 h 08 min | 12 h 15 min |
| 3 mg | 10 mM | < 1 min | 5 h 15 min | 0 h 06 min | 0 h 47 min | 1 h 32 min |
| 6 mg | 20 mM | < 1 min | 1 h 16 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 19 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 12 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A400c Agilent DD2 400 MHz NMR Spectrometer (Autosampler)

**Short Name:** A400c

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 3

**Date of Acquisition:** October 2013

\

2 channel system with AS7620 96-sample changer. OneNMR probe with auto-tune-and-match installed.

Primarily used for short, routine NMR experiments where data is needed quickly.

[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)

Best experiments:

- PROTON
- COSY
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)

Acceptable experiments:

- C13CPD (night time)
- 2D HSQC and HMBC experiments (night time)
- 1D/2D NOE experiments (night time)

Not available:

- Variable Temperature experiments
- Kinetics experiments
- Simultaneous F19 and H1 experiments

**Time Required for NMR Experiments on A400c:**

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 10 h 40 min | 35389 hours | 1785 hours | 1886 hours | 7129 hours |
| 0.06 mg | 0.2 mM | 2 h 40 min | 10516 hours | 46 h 28 min | 474 h 12 min | 1785 hours |
| 0.15 mg | 0.5 mM | 0 h 26 min | 1415 h 33 min | 6 h 10 min | 75 h 01 min | 284 h 44 min |
| 0.3 mg | 1 mM | 0 h 06 min | 354 h 01 min | 1 h 40 min | 18 h 53 min | 71 h 19 min |
| 0.6 mg | 2 mM | 0 h 01 min | 88 h 31 min | 0 h 26 min | 4 h 42 min | 17 h 51 min |
| 1.5 mg | 5 mM | < 1 min | 14 h 09 min | 0 h 03 min | 0 h 45 min | 2 h 51 min |
| 3 mg | 10 mM | < 1 min | 3 h 32 min | 0 h 03 min | 0 h 16 min | 1 h 07 min |
| 6 mg | 20 mM | < 1 min | 0 h 53 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 13 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 08 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A500a Agilent DD2 500 MHz NMR Spectrometer (Manual)

**Short Name:** A500a

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 3

**Date of Acquisition:** January 2014

\

2 channel system, OneNMR probe with auto-tune-and-match installed.

[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)

Best experiments:

- PROTON
- COSY
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
- Variable Temperature experiments
- Kinetics experiments

Acceptable experiments:

- C13CPD

Not available:

- Simultaneous F19 and H1 experiments

**Time Required for NMR Experiments on A500a:**

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 5 hours | 16962 hours | 67 h 16 min | 820 h 42 min | 3113 h 37 min |
| 0.06 mg | 0.2 mM | 1 h 10 min | 424 h 02 min | 16 h 50 min | 205 h 15 min | 779 h 06 min |
| 0.15 mg | 0.5 mM | 0 h 11 min | 678 h 28 min | 2 h 45 min | 32 h 50 min | 124 h 34 min |
| 0.3 mg | 1 mM | 0 h 03 min | 170 h 00 min | 0 h 42 min | 8 h 20 min | 31 h 09 min |
| 0.6 mg | 2 mM | < 1 min | 4 h 15 min | 0 h 11 min | 2 h 05 min | 7 h 48 min |
| 1.5 mg | 5 mM | < 1 min | 3 h 11 min | 0 h 03 min | 0 h 08 min | 1 h 40 min |
| 3 mg | 10 mM | < 1 min | 1 h 42 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 25 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 06 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A500b Agilent DD2 500 MHz NMR Spectrometer (Manual)

**Short Name:** A500b

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 3

**Date of Acquisition:** September 2012

\

2 channel system, OneNMR probe with auto-tune-and-match installed.

[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)

Best experiments:

- PROTON
- COSY
- 2D HSQC and HMBC experiments
- 1D/2D NOE experiments
- Other nuclei: F19 (no H1 decoupling), P31, B11, (others by request)
- Variable Temperature experiments
- Kinetics experiments

Acceptable experiments:

- C13CPD

Not available:

- Simultaneous F19 and H1 experiments

**Time Required for NMR Experiments on A500b:**

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 5 hours | 16962 hours | 67 h 16 min | 820 h 42 min | 3113 h 37 min |
| 0.06 mg | 0.2 mM | 1 h 10 min | 424 h 02 min | 16 h 50 min | 205 h 15 min | 779 h 06 min |
| 0.15 mg | 0.5 mM | 0 h 11 min | 678 h 28 min | 2 h 45 min | 32 h 50 min | 124 h 34 min |
| 0.3 mg | 1 mM | 0 h 03 min | 170 h 00 min | 0 h 42 min | 8 h 20 min | 31 h 09 min |
| 0.6 mg | 2 mM | < 1 min | 4 h 15 min | 0 h 11 min | 2 h 05 min | 7 h 48 min |
| 1.5 mg | 5 mM | < 1 min | 3 h 11 min | 0 h 03 min | 0 h 08 min | 1 h 40 min |
| 3 mg | 10 mM | < 1 min | 1 h 42 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 25 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 06 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A600a Agilent DD2 600 MHz NMR Spectrometer (Autosampler)

**Short Name:** A600a

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 3

**Date of Acquisition:** October 2013

\

3 channel system with AS7510 12-sample automatic sample changer. C{H} cold probe with auto-tune-and-match installed. Highest sensitivity for C13 NMR experiments.

[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)

Best experiments:

- CARBON
- PROTON

Acceptable experiments:

- 1D/2D NOE experiments

Not available:

- Variable Temperature experiments
- Kinetics experiments
- Nuclei other than H1 and C13

**Time Required for NMR Experiments on A600a:**

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 0 h 24 min | 1298 hours | 5 h 54 min | 72 h 04 min | 272 h 22 min |
| 0.06 mg | 0.2 mM | 0 h 06 min | 324 h 32 min | 1 h 28 min | 19 h 35 min | 68 h 32 min |
| 0.15 mg | 0.5 mM | 0 h 01 min | 51 h 56 min | 0 h 16 min | 3 h 11 min | 11 h 10 min |
| 0.3 mg | 1 mM | < 1 min | 13 h 00 min | 0 h 03 min | 0 h 47 min | 2 h 47 min |
| 0.6 mg | 2 mM | < 1 min | 3 h 15 min | 0 h 03 min | 0 h 16 min | 1 h 07 min |
| 1.5 mg | 5 mM | < 1 min | 0 h 31 min | 0 h 03 min | 0 h 08 min | 0 h 34 min |
| 3 mg | 10 mM | < 1 min | 0 h 08 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

A800 Agilent 800 MHz NMR Spectrometer (Manual)

**Short Name:** A800

**Instrument Type:** NMR & EPR

**Instrument Location:** CRB 100

**Date of Acquisition:** July 2013

\

4 channel system. Inverse-detect HCN probe, Dual Band (direct-detect) probe, and H{CN} C13-sensitivity enhanced salt-tolerant cold probe available.

Experienced Users Only. Contact CBIC staff for more information.

EPR Bruker EleXsys EPR Spectrometer

**Short Name:** EPR

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 12

**Date of Acquisition:** September 2000

\

X-Band Electron Paramagnetic Resonance. Equipped with a cryogen-free cryostat.

V600a Varian VNMRS 600 MHz NMR Spectrometer (Manual)

**Short Name:** V600a

**Instrument Type:** NMR & EPR

**Instrument Location:** CRB 100

**Date of Acquisition:** July 2009

\

3 channel system. Inverse-detect HCN probe installed.

V600b Varian VNMRS 600 MHz NMR Spectrometer (Manual)

**Short Name:** V600b

**Instrument Type:** NMR & EPR

**Instrument Location:** CRB 100

**Date of Acquisition:** August 2002

\

3 channel system. Inverse-detect HCN probe installed.

V700 Varian Inova 700 MHz NMR Spectrometer (Manual)

**Short Name:** V300b

**Instrument Type:** NMR & EPR

**Instrument Location:** KCL 3

**Date of Acquisition:** October 2010

\

4 channel system, triple resonance probe normally installed.