Yale University

Source: https://cbic.yale.edu/instruments-view/a600a Parent: https://cbic.yale.edu/instruments/experiment-runtime-estimation

A600a Agilent DD2 600 MHz NMR Spectrometer (Autosampler)

Short Name:

A600a

Instrument Type:

NMR & EPR

Location:

KCL 3

Date of Acquisition:

October 2013

Description:

3 channel system with AS7510 12-sample automatic sample changer. C{H} cold probe with auto-tune-and-match installed. Highest sensitivity for C13 NMR experiments.

Guides and Standard Operating Procedures

Best experiments:

CARBON
PROTON

Acceptable experiments:

1D/2D NOE experiments

Not available:

Variable Temperature experiments
Kinetics experiments
Nuclei other than H1 and C13

Time Required for NMR Experiments on A600a:


Mass	Concentration	Proton	Carbon	gCOSY	gHSQC	gHMBC
0.03 mg	0.1 mM	0 h 24 min	1298 hours	5 h 54 min	72 h 04 min	272 h 22 min
0.06 mg	0.2 mM	0 h 06 min	324 h 32 min	1 h 28 min	19 h 35 min	68 h 32 min
0.15 mg	0.5 mM	0 h 01 min	51 h 56 min	0 h 16 min	3 h 11 min	11 h 10 min
0.3 mg	1 mM	< 1 min	13 h 00 min	0 h 03 min	0 h 47 min	2 h 47 min
0.6 mg	2 mM	< 1 min	3 h 15 min	0 h 03 min	0 h 16 min	1 h 07 min
1.5 mg	5 mM	< 1 min	0 h 31 min	0 h 03 min	0 h 08 min	0 h 34 min
3 mg	10 mM	< 1 min	0 h 08 min	0 h 03 min	0 h 08 min	0 h 17 min
6 mg	20 mM	< 1 min	0 h 04 min	0 h 03 min	0 h 08 min	0 h 17 min
12 mg	40 mM	< 1 min	0 h 04 min	0 h 03 min	0 h 08 min	0 h 17 min
15 mg	50 mM	< 1 min	0 h 04 min	0 h 03 min	0 h 08 min	0 h 17 min
> 30 mg	100+ mM	< 1 min	0 h 04 min	0 h 03 min	0 h 08 min	0 h 17 min

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

Instrument Image: