# Yale University

**Source**: https://cbic.yale.edu/instruments-view/a600a
**Parent**: https://cbic.yale.edu/instruments/experiment-runtime-estimation

# A600a Agilent DD2 600 MHz NMR Spectrometer (Autosampler)

Short Name:

A600a

Instrument Type:

NMR & EPR

Location:

KCL 3

Date of Acquisition:

October 2013

Description:

3 channel system with AS7510 12-sample automatic sample changer. C{H} cold probe with auto-tune-and-match installed. Highest sensitivity for C13 NMR experiments.

[Guides and Standard Operating Procedures](https://cbic.yale.edu/training/user-guides/nmr)

Best experiments:

- CARBON
- PROTON

Acceptable experiments:

- 1D/2D NOE experiments

Not available:

- Variable Temperature experiments
- Kinetics experiments
- Nuclei other than H1 and C13

**Time Required for NMR Experiments on A600a:**

|  |  |  |  |  |  |  |
| --- | --- | --- | --- | --- | --- | --- |
| Mass | Concentration | Proton | Carbon | gCOSY | gHSQC | gHMBC |
| 0.03 mg | 0.1 mM | 0 h 24 min | 1298 hours | 5 h 54 min | 72 h 04 min | 272 h 22 min |
| 0.06 mg | 0.2 mM | 0 h 06 min | 324 h 32 min | 1 h 28 min | 19 h 35 min | 68 h 32 min |
| 0.15 mg | 0.5 mM | 0 h 01 min | 51 h 56 min | 0 h 16 min | 3 h 11 min | 11 h 10 min |
| 0.3 mg | 1 mM | < 1 min | 13 h 00 min | 0 h 03 min | 0 h 47 min | 2 h 47 min |
| 0.6 mg | 2 mM | < 1 min | 3 h 15 min | 0 h 03 min | 0 h 16 min | 1 h 07 min |
| 1.5 mg | 5 mM | < 1 min | 0 h 31 min | 0 h 03 min | 0 h 08 min | 0 h 34 min |
| 3 mg | 10 mM | < 1 min | 0 h 08 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 6 mg | 20 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 12 mg | 40 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| 15 mg | 50 mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |
| > 30 mg | 100+ mM | < 1 min | 0 h 04 min | 0 h 03 min | 0 h 08 min | 0 h 17 min |

All estimates based on a 500 MW sample in 600 uL solvent volume using a 5 mm diameter NMR tube

Instrument Image: