Engineered surfaces that promote capture of latent proteins to facilitate integrin-mediated mechanical activation of growth factors
Source: https://eprints.gla.ac.uk/316746/ Parent: https://eprints.gla.ac.uk/view/project_code/315918.html
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Engineered surfaces that promote capture of latent proteins to facilitate integrin-mediated mechanical activation of growth factors
Dhawan, Udesh ORCID: https://orcid.org/0000-0002-0393-9414, Williams, Jonathan A., Windmill, James F.C., Childs, Peter, Gonzalez-Garcia, Cristina, Dalby, Matthew J. ORCID: https://orcid.org/0000-0002-0528-3359 and Salmeron-Sanchez, Manuel ORCID: https://orcid.org/0000-0002-8112-2100 (2024) Engineered surfaces that promote capture of latent proteins to facilitate integrin-mediated mechanical activation of growth factors. Advanced Materials, 36(23), 2310789. (doi: 10.1002/adma.202310789) (PMID:38253339)
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Abstract
Conventional osteogenic platforms utilize active growth factors to repair bone defects that are extensive in size, but they can adversely affect patient health. Here, an unconventional osteogenic platform is reported that functions by promoting capture of inactive osteogenic growth factor molecules to the site of cell growth for subsequent integrin-mediated activation, using a recombinant fragment of latent transforming growth factor beta-binding protein-1 (rLTBP1). It is shown that rLTBP1 binds to the growth-factor- and integrin-binding domains of fibronectin on poly(ethyl acrylate) surfaces, which immobilizes rLTBP1 and promotes the binding of latency associated peptide (LAP), within which inactive transforming growth factor beta 1 (TGF-β1) is bound. rLTBP1 facilitates the interaction of LAP with integrin β1 and the subsequent mechanically driven release of TGF-β1 to stimulate canonical TGF-β1 signaling, activating osteogenic marker expression in vitro and complete regeneration of a critical-sized bone defect in vivo.
| Item Type: | Articles |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Salmeron-Sanchez, Professor Manuel and Windmill, Professor James and Dhawan, Dr Udesh and Childs, Dr Peter and Dalby, Professor Matthew and Gonzalez Garcia, Dr Cristina |
| Authors: | Dhawan, U., Williams, J. A., Windmill, J. F.C., Childs, P., Gonzalez-Garcia, C., Dalby, M. J., and Salmeron-Sanchez, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Science and Engineering > School of Engineering > Biomedical Engineering |
| Journal Name: | Advanced Materials |
| Publisher: | Wiley |
| ISSN: | 0935-9648 |
| ISSN (Online): | 1521-4095 |
| Published Online: | 22 January 2024 |
| Copyright Holders: | Copyright © 2024 The Authors |
| First Published: | First published in Advanced Materials 36(23): 2310789 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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Funder and Project Information
Funder and Project Information
Funder and Project Information
Project Code
Award No
Project Name
Principal Investigator
Funder's Name
Funder Ref
Lead Dept
HEALIKICK
Manuel Salmeron-Sanchez
874889
School of Molecular Biosciences
DEVISE - Engineered viscoelasticity in regenerative microenvironments
Manuel Salmeron-Sanchez
101054728
ENG - Biomedical Engineering
Bioactive polysaccharide-based hydrogels for growth factors delivery during tissue repair.
Cristina Gonzalez Garcia
Engineering and Physical Sciences Research Council (EPSRC)
EP/T000457/1
ENG - Biomedical Engineering
Deposit and Record Details
| ID Code: | 316746 |
| Depositing User: | Mr Alastair Arthur |
| Datestamp: | 23 Jan 2024 16:20 |
| Last Modified: | 29 Oct 2024 09:51 |
| Date of acceptance: | 15 January 2024 |
| Date of first online publication: | 22 January 2024 |
| Date Deposited: | 6 February 2024 |
| Data Availability Statement: | Yes |
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