# Engineered surfaces that promote capture of latent proteins to facilitate integrin-mediated mechanical activation of growth factors
**Source**: https://eprints.gla.ac.uk/316746/
**Parent**: https://eprints.gla.ac.uk/view/project_code/315918.html
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# Engineered surfaces that promote capture of latent proteins to facilitate integrin-mediated mechanical activation of growth factors
[Dhawan, Udesh](https://eprints.gla.ac.uk/view/author/61603.html) [ORCID: https://orcid.org/0000-0002-0393-9414](https://orcid.org/0000-0002-0393-9414), Williams, Jonathan A., [Windmill, James F.C.](https://eprints.gla.ac.uk/view/author/54034.html), [Childs, Peter](https://eprints.gla.ac.uk/view/author/25713.html), [Gonzalez-Garcia, Cristina](https://eprints.gla.ac.uk/view/author/31180.html), [Dalby, Matthew J.](https://eprints.gla.ac.uk/view/author/9268.html) [ORCID: https://orcid.org/0000-0002-0528-3359](https://orcid.org/0000-0002-0528-3359) and [Salmeron-Sanchez, Manuel](https://eprints.gla.ac.uk/view/author/30067.html) [ORCID: https://orcid.org/0000-0002-8112-2100](https://orcid.org/0000-0002-8112-2100)
(2024)
Engineered surfaces that promote capture of latent proteins to facilitate integrin-mediated mechanical activation of growth factors.
*[Advanced Materials](https://eprints.gla.ac.uk/view/journal_volume/Advanced_Materials.html)*, 36(23),
2310789.
(doi: [10.1002/adma.202310789](https://doi.org/10.1002/adma.202310789))
(PMID:[38253339](https://europepmc.org/abstract/MED/38253339))
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| | [Text](https://eprints.gla.ac.uk/316746/1/316746.pdf) 316746.pdf - Published Version Available under License [Creative Commons Attribution](http://creativecommons.org/licenses/by/4.0/). 5MB |
## Abstract
Conventional osteogenic platforms utilize active growth factors to repair bone defects that are extensive in size, but they can adversely affect patient health. Here, an unconventional osteogenic platform is reported that functions by promoting capture of inactive osteogenic growth factor molecules to the site of cell growth for subsequent integrin-mediated activation, using a recombinant fragment of latent transforming growth factor beta-binding protein-1 (rLTBP1). It is shown that rLTBP1 binds to the growth-factor- and integrin-binding domains of fibronectin on poly(ethyl acrylate) surfaces, which immobilizes rLTBP1 and promotes the binding of latency associated peptide (LAP), within which inactive transforming growth factor beta 1 (TGF-β1) is bound. rLTBP1 facilitates the interaction of LAP with integrin β1 and the subsequent mechanically driven release of TGF-β1 to stimulate canonical TGF-β1 signaling, activating osteogenic marker expression in vitro and complete regeneration of a critical-sized bone defect in vivo.
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| Item Type: | Articles |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | [Salmeron-Sanchez, Professor Manuel](https://eprints.gla.ac.uk/view/author/30067.html) and [Windmill, Professor James](https://eprints.gla.ac.uk/view/author/54034.html) and [Dhawan, Dr Udesh](https://eprints.gla.ac.uk/view/author/61603.html) and [Childs, Dr Peter](https://eprints.gla.ac.uk/view/author/25713.html) and [Dalby, Professor Matthew](https://eprints.gla.ac.uk/view/author/9268.html) and [Gonzalez Garcia, Dr Cristina](https://eprints.gla.ac.uk/view/author/31180.html) |
| Authors: | [Dhawan, U.](https://eprints.gla.ac.uk/view/author/61603.html), Williams, J. A., [Windmill, J. F.C.](https://eprints.gla.ac.uk/view/author/54034.html), [Childs, P.](https://eprints.gla.ac.uk/view/author/25713.html), [Gonzalez-Garcia, C.](https://eprints.gla.ac.uk/view/author/31180.html), [Dalby, M. J.](https://eprints.gla.ac.uk/view/author/9268.html), and [Salmeron-Sanchez, M.](https://eprints.gla.ac.uk/view/author/30067.html) |
| College/School: | [College of Medical Veterinary and Life Sciences](https://eprints.gla.ac.uk/view/divisions/20000000/) > [School of Molecular Biosciences](https://eprints.gla.ac.uk/view/divisions/25400000/) [College of Science and Engineering](https://eprints.gla.ac.uk/view/divisions/30000000/) > [School of Engineering](https://eprints.gla.ac.uk/view/divisions/30300000/) > [Biomedical Engineering](https://eprints.gla.ac.uk/view/divisions/30303000/) |
| Journal Name: | [Advanced Materials](https://eprints.gla.ac.uk/view/journal_volume/Advanced_Materials.html) |
| Publisher: | Wiley |
| ISSN: | 0935-9648 |
| ISSN (Online): | 1521-4095 |
| Published Online: | 22 January 2024 |
| Copyright Holders: | Copyright © 2024 The Authors |
| First Published: | First published in Advanced Materials 36(23): 2310789 |
| Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: [Request a correction](https://eprints.gla.ac.uk/cgi/request_correction?eprintid=316746) | Enlighten Editors: [Update this record](https://eprints.gla.ac.uk/cgi/users/home?screen=EPrint::View&eprintid=316746)
Funder and Project Information
[Funder and Project Information](#)
[Funder and Project Information](#)
Project Code
Award No
Project Name
Principal Investigator
Funder's Name
Funder Ref
Lead Dept
[307621](https://eprints.gla.ac.uk/view/project_code/307621.html)
HEALIKICK
Manuel Salmeron-Sanchez
[European Commission (EC)](https://eprints.gla.ac.uk/view/funder/European_Commission_=28EC=29.html)
874889
School of Molecular Biosciences
[315918](https://eprints.gla.ac.uk/view/project_code/315918.html)
DEVISE - Engineered viscoelasticity in regenerative microenvironments
Manuel Salmeron-Sanchez
[EPSRC EU Guarantee (EPSRCEU)](https://eprints.gla.ac.uk/view/funder/EPSRC_EU_Guarantee_=28EPSRCEU=29.html)
101054728
ENG - Biomedical Engineering
[304627](https://eprints.gla.ac.uk/view/project_code/304627.html)
Bioactive polysaccharide-based hydrogels for growth factors delivery during tissue repair.
Cristina Gonzalez Garcia
[Engineering and Physical Sciences Research Council (EPSRC)](https://eprints.gla.ac.uk/view/funder/Engineering_and_Physical_Sciences_Research_Council_=28EPSRC=29.html)
EP/T000457/1
ENG - Biomedical Engineering
Deposit and Record Details
[Deposit and Record Details](#)
[Deposit and Record Details](#)
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| ID Code: | 316746 |
| Depositing User: | [Mr Alastair Arthur](https://eprints.gla.ac.uk/profile/29814) |
| Datestamp: | 23 Jan 2024 16:20 |
| Last Modified: | 29 Oct 2024 09:51 |
| Date of acceptance: | 15 January 2024 |
| Date of first online publication: | 22 January 2024 |
| Date Deposited: | 6 February 2024 |
| Data Availability Statement: | Yes |
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